Evidence map›Paper›PMID 40531458›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Tips and Tricks in Reversed-Phase Flash Chromatography for Peptide Purification.

Elizabeth Denton, Amit Mehrotra

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Improvement of Analysis and Transferability in Peptide Purification: From HPLC to FPLC and Back Again.Journal of peptide science : an official publication of the European Peptide Society · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Elizabeth DentonBiotage, LLC, Charlotte, NC, USA.
Amit MehrotraBiotage AB, Uppsala, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purification of peptides produced by chemical synthesis or semi-synthesis is required nearly for all sequences. Reversed-phase high pressure (performance) liquid chromatography (RP-HPLC) has long been the purification method of choice for these molecules. But as solid phase synthesis strategies have improved and demand for increasing numbers of peptides continues, alternative purification options are now being developed and employed in the traditional peptide synthesis workflow. Herein we introduce reversed-phase high performance flash chromatography (RP-HPFC) and describe strategies enabling its use as a sole or complimentary purification strategy for traditional RP-HPLC methodologies.

Indexed as

Chromatography, Reverse-PhasePeptidesChromatography, High Pressure LiquidSolid-Phase Synthesis TechniquesPeptidesHigh performance flash chromatographyPeptide nucleic acidsPeptidesPeptidomimeticsPreparative ChromatographyReversed-phase purification

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.