Evidence map›Paper›PMID 40531435›Full record

ArticleTransgenic research2025

Agrobacterium-mediated genetic transformation of chia (Salvia hispanica L.), a rich source of omega-3 fatty acid.

N Anirudh, P K Haritha, R V Sreedhar

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Functional Analysis of Naturally IntegratedPlants (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

N AnirudhPlant Cell Biotechnology Department, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysuru, Karnataka, 570020, India.
P K HarithaPlant Cell Biotechnology Department, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysuru, Karnataka, 570020, India.
R V SreedharPlant Cell Biotechnology Department, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysuru, Karnataka, 570020, India. rvs@cftri.res.in.

Funding

Science and Engineering Research Board, DST, India ECR/2018/002370
6 · The paper itself

Abstract

Chia (Salvia hispanica L.), a nutritionally valuable crop, is the richest source of α-linolenic acid, a key omega-3 fatty acid. Despite its nutritional benefits, a stable genetic transformation method for chia is not available. This study presents a sonication-assisted Agrobacterium-mediated transformation protocol optimized for high-efficiency transformation of chia seedlings. Key parameters including bacterial cell density, acetosyringone concentration, sonication duration, vacuum infiltration, and infection time were optimized. Results demonstrated that an optical density at 600 nm (OD

Indexed as

AgrobacteriumFatty Acids, Omega-3Plants, Genetically ModifiedSalviaTransformation, GeneticAcetophenonesAgrobacterium tumefaciensSeedlingsAcetophenonesacetosyringoneFatty Acids, Omega-3Agrobacterium tumefaciensGenetic transformationGUSSalvia hispanica L.Transgenic plant

Identifiers

PMID40531435

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.