ArticleNucleic acids research2025
XPC-RAD23B enhances UV-DDB binding to DNA to facilitate lesion search in nucleotide excision repair.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- DNA Repair Mechanisms.Methods in molecular biology (Clifton, N.J.) · 2027Review
- Assessment and detection of nucleotide excision repair capability in human cells: a critical review of current functional methods.Molecular biology reports · 2026Review
- Beyond the Surface: Mechanistic Intersections Between Obesity and UVB Exposure.International journal of obesity (2005) · 2026Review
- Engineering quantum dot surfaces to preserve protein-DNA interactions for single-molecule visualization.Nano convergence · 2026Article
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Authors and funding
11 authors.
Funding
Abstract
Ultraviolet-induced DNA lesions are removed by the nucleotide excision repair (NER) pathway. In global-genome NER (GG-NER), XPC-RAD23B recognizes the lesions and initiates NER. However, cyclobutane pyrimidine dimers (CPDs), which do not significantly destabilize the DNA duplex, are not bound by XPC-RAD23B with high selectivity. Instead, CPD is preferentially sensed by UV-DDB, which is believed to hand over the lesion to XPC-RAD23B via ubiquitination of both proteins. Here, by combining biochemical and single-molecule DNA curtain assays, we investigate the interactions between UV-DDB and XPC-RAD23B on DNA. Surprisingly, we discover that XPC-RAD23B enhances the binding of UV-DDB to DNA. We demonstrate that this enhancement can be attributed to the complex formation of UV-DDB and XPC-RAD23B (UX-complex), which increases the binding affinity of UV-DDB to undamaged DNA. We further show that UV-DDB finds CPDs through one-dimensional (1D) diffusion along DNA. Collectively, the UX-complex enhances UV-DDB loading to DNA to accelerate the search for CPD via 1D diffusion. Moreover, we find that UV-DDB and XPC-RAD23B can bind CPDs as a complex, which facilitates the transfer of CPD. Altogether, our results show that UV-DDB and XPC-RAD23B cooperatively interact for rapid CPD search, providing a new mechanism for lesion search in GG-NER.
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