Evidence map›Paper›PMID 40528429›Full record

ArticleAnnals of medicine2025

Da-Yuan-Yin decoction suppresses NETs formation, inhibits the IL-23/JAK2/STAT3 signalling pathway, and modulates metabolic profiles and gut microbiota composition in damp-heat syndrome.

Yun Yang, Lengqiu Guo, Jinghua Yu, Wei Peng, Qi Wang, Lan Wei, Xihua Chen, Chunyan Min, Xiaogang Jiang, Song Zhu and 4 more

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PubMed Full text at PMC Publisher
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Article in Annals of medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Trial
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yun YangCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Lengqiu GuoJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Suzhou, China.
Jinghua YuCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Wei PengDepartment of the Gastrointestinal Surgery, Second Affiliated Hospital of Soochow University, Suzhou, China.
Qi WangJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Suzhou, China.
Lan WeiCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Xihua ChenCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Chunyan MinPharmaceutical Standards Laboratory, Suzhou Institute for Drug Control, Suzhou, China.
Xiaogang JiangCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Song ZhuCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Haixin ZhaoSchool of International Studies, Shaanxi Normal University, Xian, China.
Zhenyu XuanCollege of Pharmaceutical Science, Soochow University, Suzhou, China.
Jianzhong YuDepartment of Internal Medicine of Traditional Chinese Medicine, Haian Hospital of Traditional Chinese Medicine, Nantong, China.
Jian ZhangCollege of Pharmaceutical Science, Soochow University, Suzhou, China.ORCID 0000-0002-4372-2826

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDa-Yuan-Yin Decoction (DYY), used since the early Qing Dynasty in China for damp-heat syndrome, was evaluated for its comprehensive protective effects. MATERIALS AND

methodsRats received a high-fat/high-sugar diet for 14 days, then daily exposure to high temperature/humidity (31.0 ± 0.5°C, 85%-95%) for 10 hours over 14 days. Damp-heat syndrome was induced on day 29 via intraperitoneal lipopolysaccharide (LPS) injections (1 mg/kg and 0.5 mg/kg after 4 hours). Two hours post-modeling, rats received DYY (400 or 800 mg/kg/day) orally for 5 days. Symptoms were scored. On day 33, kidney, spleen, tongue, lung, liver, stomach, and colon tissues underwent histopathological and immunochemical/immunofluorescence analysis. Serum levels of inflammation factors, C-reactive protein (CRP), cell-free DNA (cfDNA), myeloperoxidase (MPO), and superoxide dismutase (SOD) were quantified. Complete blood count (CBC), blood biochemistry, short-chain fatty acids (SCFAs), gut microbiota composition in the colon, and metabolites in the cecum were analyzed.

resultsDYY significantly reduced symptom scores and decreased CRP level, while also regulating parameters in CBC and biochemical profiles. The treatment ameliorated tissue damage in the kidney, spleen, tongue, lung, liver, stomach, and colon. DYY downregulated citrullinated histone H3 (Cit H3), polyclonal peptidylarginine deiminase 4 (PADI4), and MPO expressions in the lung, stomach, and colon. Furthermore, it modulated inflammatory responses, suppressed the interleukin-23 (IL-23)/janus kinase-2 (JAK2)/signal transduction and transcription activator-3 (STAT3) signaling pathway and enhanced antioxidant activity. DYY administration significantly increased total SCFA content. The intervention restored gut microbiota diversity and abundance, while ameliorating metabolic dysregulation.

conclusionSystemic pathophysiological alterations were observed in rats with damp-heat syndrome. The comprehensive protective effects of DYY involved suppression of neutrophil extracellular traps (NETs) formation through downregulation of PADI4 expression, modulation of inflammatory responses, inhibition of the IL-23/JAK2/STAT3 signaling pathway, restoration of intestinal microecological balance and metabolic homeostasis, and enhancement of antioxidant.

Indexed as

Drugs, Chinese HerbalExtracellular TrapsHeat ExhaustionHot TemperatureHumidityAnimalsDietary SugarsDiet, High-FatDisease Models, AnimalGastrointestinal MicrobiomeInterleukin-23Janus Kinase 2LipopolysaccharidesMaleRatsRats, Sprague-DawleyDietary SugarsDrugs, Chinese HerbalInterleukin-23Janus Kinase 2LipopolysaccharidesSTAT3 Transcription Factordamp-heat syndromeDa-Yuan-Yin decoctionfaecal metabolitesgut microbiotainflammationneutrophil extracellular traps

Identifiers

PMID40528429
PMCPMC12180317

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.