ArticleACS biomaterials science & engineering2025
Scaffolding Light-Up Aptamers on DNA Nanostructures for Fluorescence Enhancement.
Article in ACS biomaterials science & engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Prospects of DNA nanotechnology in stroke repair and regeneration.Communications biology · 2026Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
RNA-based fluorescent light-up aptamers (FLAPs) have been progressively developed as imaging probes because of their high signal-to-noise ratio. However, it remains a challenge to use these light-up aptamers due to their poor folding and stability. Leveraging DNA nanotechnology, we investigated whether a DNA origami template could improve folding and further enhance the functionality of FLAPs, namely, the corresponding fluorescence intensities. We utilized aptamer Broccoli and its cognate fluorogen DFHBI-1T as a model. When singular aptamer Broccoli was scaffolded on DNA origami, DNA brick-based nanostructures, DNA double helices, and even on structures as simple as a DNA hairpin stem, our results showed that the fluorescence intensities could be significantly enhanced. These findings show a positive correlation between the fluorogen activity of light-up aptamers and the DNA stem length, potentially mediated by the improved structural stability of the DNA stem, as determined by their simulated thermodynamic properties. Our studies provide a new method to design and enhance the fluorescence behavior of FLAPs, especially structures with a G-quadruplex-based fluorogen recognition region.
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Registered trials
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