Evidence map›Paper›PMID 40528093›Full record

ArticleMolecular biology reports2025

Exploring microbial diversity in the Kharasinpur hot spring of West Bengal, India.

Rakesh Goswami, Aniket Sarkar, Bidyut Bandyopadhyay, Sanjoy Sadhukhan

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Rakesh GoswamiDepartment of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore, West Bengal, India.ORCID http://orcid.org/0000-0003-1590-6211
Aniket SarkarDepartment of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore, West Bengal, India.ORCID http://orcid.org/0000-0002-8063-020X
Bidyut BandyopadhyayDepartment of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore, West Bengal, India.ORCID http://orcid.org/0000-0003-4472-6252
Sanjoy SadhukhanPlant Molecular Biology Laboratory, Department of Botany, Raiganj University, Uttar Dinajpur, Raiganj, West Bengal, 733134, India. sanjoysadhukhan@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHot springs are natural geothermal environments that harbour thermophilic microorganisms with significant biotechnological potential. Despite extensive studies on many Indian hot springs, Kharasinpur Hot Spring in West Bengal remains totally unexplored in terms of microbial diversity and physicochemical properties.

methodsA total of seven bacterial isolates were obtained from water samples collected at the Kharasinpur Hot Spring. These isolates underwent morphological, physiological, biochemical, and molecular characterization through 16S rRNA gene sequencing. Water samples were analyzed for physicochemical parameters such as temperature, pH, total hardness, iron concentration, salt content, and total dissolved solids (TDS).

resultsThe water temperature was recorded at 65 °C with a slightly alkaline pH (7.24). The 16S rRNA analysis identified isolates belonging to the phyla Pseudomonadota (e.g., Pseudomonas sp., Hydrogenophaga sp.) and Bacillota (e.g., Staphylococcus sp., Neobacillus sp.). The high iron content in the hot spring water was notable and rendered it unsafe for direct human consumption.

conclusionThis study provides novel insights into the microbial diversity and physicochemical characteristics of a relatively unstudied Indian hot spring. The findings contribute to broader research efforts on hot spring ecosystems in India, enhancing our understanding of their microbial ecology and potential health implications.

Indexed as

BacteriaHot SpringsBiodiversityHydrogen-Ion ConcentrationIndiaIronMicrobiotaPhylogenyRNA, Ribosomal, 16SWater MicrobiologyIronRNA, Ribosomal, 16SHot springKharasinpurPseudomonadotaThermophiles

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