ArticleJournal of the Royal Society, Interface2025
Viscoelastic differences between isolated and live MCF7 cancer cell nuclei resolved with AFM microrheology.
Article in Journal of the Royal Society, Interface, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- Viscoelastic differences between isolated and live MCF7 cancer cell nuclei resolved with AFM microrheology.Journal of the Royal Society, Interface · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Cell nuclei are commonly isolated for mechanobiology studies although isolated nuclei may display viscoelastic properties differing from those of live cells. Nuclear mechanics is generally dependent on the time scale of the applied load and cannot accurately be assessed by a simple elasticity parameter. Active microrheology with an atomic force microscope (AFMMR) is a versatile tool for probing nuclear mechanics and we employ the technique for exploring isolated and live-cell nuclei in MCF7 cells, including the significance of actin depolymerization. We successfully validate the method using polyacrylamide hydrogels with correction for cantilever drag in the fluid. The AFMMR results reveal that isolated and live-cell nuclei are equivalent to within a scaling factor, in their frequency-dependent modulus, with isolated nuclei being softer. The loss tangent reveals a transition from solid- to liquid-like behaviour occurring at higher frequency in isolated than in live-cell nuclei. Viscoelastic modelling using the Jeffreys model describes the frequency-dependent modulus of all measured nuclei. Model parameters display sensitivity to nuclei isolation and to actin depolymerization in live cells. Sections of the Jeffreys circuit can potentially be assigned to internal and external nucleus structures, respectively, thereby establishing a minimal mechanistic framework for interpreting microrheology data on cell nuclei.
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Registered trials
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