Evidence map›Paper›PMID 40527155›Full record

ReviewCurrent opinion in structural biology2025

Elucidating double stranded DNA viral scaffolding protein structures through advances in cryogenic electron microscopy data processing.

Makayla N Leroux, Garrett S Skidds, Carolyn M Teschke

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Current opinion in structural biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Makayla N LerouxDepartment of Molecular and Cell Biology, University of Connecticut, 91 N. Eagleville Rd, Storrs, CT, 06269-3125, USA.
Garrett S SkiddsDepartment of Molecular and Cell Biology, University of Connecticut, 91 N. Eagleville Rd, Storrs, CT, 06269-3125, USA.
Carolyn M TeschkeDepartment of Molecular and Cell Biology, University of Connecticut, 91 N. Eagleville Rd, Storrs, CT, 06269-3125, USA; Department of Chemistry, University of Connecticut, 55 N. Eagleville Rd, Storrs, CT, 06269-3060, USA. Electronic address: carolyn.teschke@uconn.edu.

Funding

Understanding the Protein:Protein Interactions Required for Virus AssemblyR01GM076661 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI TESCHKE, CAROLYN M · 2007 to 2022
$7.2M
Investigating the conformational changes of the portal protein that drive DNA packaging in a dsDNA virusF31GM147952 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI LEROUX, MAKAYLA · 2023 to 2024
$92k
NIGMS NIH HHS F31 GM147952NIGMS NIH HHS R01 GM076661
6 · The paper itself

Abstract

Icosahedral double stranded DNA (dsDNA) virus assembly first necessitates the formation of a precursor capsid (procapsid) into which the DNA is packaged. Direct interactions between the major capsid protein (MCP) and a scaffolding protein promote proper procapsid assembly. The scaffolding protein can be an independent protein or a scaffolding-like domain covalently attached to the MCP that is comparable in structure and function. A full understanding of scaffolding protein structures has been limited by their intrinsically disordered nature. Advances in cryogenic electron microscopy (cryoEM) data processing techniques have provided new methodologies to help solve the structures of scaffolding proteins within procapsids. These structural insights further our understanding of how scaffolding proteins interact with the other assembly proteins to correctly construct the procapsid.

Indexed as

Cryoelectron MicroscopyDNA, ViralCapsidCapsid ProteinsModels, MolecularProtein ConformationCapsid ProteinsDNA, Viral

Identifiers

PMID40527155
PMCPMC12236358

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.