Evidence map›Paper›PMID 40525453›Full record

ArticleRevista espanola de salud publica2025

[Bacterial filtration efficiency (BFE) of surgical masks used for eight hours and unused masks with Staphylococcus aureus].

Sara Batista, Genoveva Cuesta, Mariana Fernandez-Pittol, Angely Roman, Griselda Tudó, Julián González-Martín

Abstract readComparative StudyEnglish Abstract
In one paragraph

Article in Revista espanola de salud publica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sara BatistaUnitat de Microbiologia, Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona. Barcelona. España.
Genoveva CuestaUnitat de Microbiologia, Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona. Barcelona. España.
Mariana Fernandez-PittolUnitat de Microbiologia, Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona. Barcelona. España.
Angely RomanServei de Microbiologia, CDB, Hospital Clínic de Barcelona. Barcelona. España.
Griselda TudóUnitat de Microbiologia, Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona. Barcelona. España.
Julián González-MartínUnitat de Microbiologia, Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona. Barcelona. España.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveSpanish regulations and World Health Organization (WHO) guidelines recommend not using surgical masks for more than four hours. The high demand and low availability of surgical masks during the COVID-19 pandemic highlighted this as a major limitation. The objective of this study was to compare the bacterial filtration efficiency (BFE) between used surgical masks (eight hours) and unused masks (zero hours).

methods208 surgical masks worn for eight hours in a row were studied. They were placed on a culture plate containing blood agar (Agar Columbia with sheep blood plus, Thermo Scientific) and sprayed a suspension of Staphylococcus aureus (ATCC 29313) at a distance of thirty centimeters. Culture plates were incubated at 37 °C for twenty-four hours. A count of colony forming units (CFU) was carried out. As a control for BFE, new non-worn masks were included. A t-test and a z-test were used at a 95% confidence level to compare the means of the two groups.

resultsTo establish the BFE, the CFU of the cultures from the used masks were compared with the control cultures without a mask. Likewise, the BFE of used masks was compared to that of new masks. The mean, median and mode values of the BFE of both masks, used and new ones, were greater than 99%. No statistically significant differences were detected between eight hours used masks and not used ones (zero hours).

conclusionsSurgical masks maintain a BFE equivalent to that new, unused surgical masks at eight hours.

Indexed as

COVID-19Equipment ContaminationMasksStaphylococcus aureusColony Count, MicrobialFiltrationHumansTime FactorsBacterial filtration efficacySARS-CoV-2 pandemicSpainStaphylococcus aureusSurgical mask

Identifiers

PMID40525453
PMCPMC12172150

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.