Evidence map›Paper›PMID 40524528›Full record

ArticleAllergy2026

Role for Complement C5 in Eosinophilic Inflammation of Severe Asthma.

Cong Dong, Shaohua Lu, Zhenan Deng, Xuliang Cai, Huahao Shen, Guochao Shi, Changxing Ou, Zuofu Peng, Wei Jiang, Xiuhua Fu and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Allergy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Cong DongState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.ORCID https://orcid.org/0000-0003-1163-7966
Shaohua LuSino-French Hoffmann Institute, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.
Zhenan DengState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Xuliang CaiState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Huahao ShenDepartment of Respiratory Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.ORCID https://orcid.org/0000-0001-9546-9351
Guochao ShiDepartment of Respiratory Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Changxing OuState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.ORCID https://orcid.org/0000-0002-9993-4617
Zuofu PengAlpha X(Beijing) Biotech CO., Beijing, China.
Wei JiangAstraZeneca, Shanghai Shi, China.
Xiuhua FuDepartment of Respiratory Medicine, The Affiliated Hospital of Inner Mongolia Medical University, Huhhot, China.
Changzheng WangDepartment of Respiratory Medicine, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Meiling JinDepartment of Respiratory Medicine, Zhongshan Hospital, Shanghai, China.
Zhongmin QiuDepartment of Pulmonary and Critical Care Medicine, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.ORCID https://orcid.org/0000-0002-6612-7413
Xiaoyang WeiDepartment of Respiratory Medicine, The Eighth Medical Center of PLA General Hospital, Beijing, China.
Wei GuDepartment of Respiratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Kewu HuangDepartment of Respiratory Medicine, Beijing Chao-Yang Hospital, Beijing, China.
Qiang LiDepartment of Respiratory Medicine, Shanghai General Hospital, Shanghai, China.
Xiangyan ZhangDepartment of Respiratory Medicine, Guizhou Province People's Hospital, Guiyang, China.
Nanshan ZhongState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Kian Fan ChungNational Heart and Lung Institute, Imperial College London, & Royal Brompton & Harefield Hospital, London, UK.ORCID https://orcid.org/0000-0001-7101-1426
Qingling ZhangState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
C‐BIOPRED Consortium

Funding

Clinical and Epidemiological Research Project of State Key Laboratory of Respiratory Disease SKLRD-L-202404Guangzhou Science and Technology Plan Project and Zhongnanshan Medical Foundation of Guangdong Province 202102010355Guangzhou Science and Technology Plan Project and Zhongnanshan Medical Foundation of Guangdong Province ZNSA-2020003Major clinical research project of the Guangzhou Medical University Research Ability Enhancement Program GMUCR2024-01010National Natural Science Foundation of China 82070026Zhongnanshan Medical Foundation of Guangdong Province ZNSA-2020013Zhongnanshan Medical Foundation of Guangdong Province ZNSXS-20220083Zhongnanshan Medical Foundation of Guangdong Province ZNSXS-20240005
6 · The paper itself

Abstract

backgroundWe investigated the role of the complement system, particularly complement C5, in severe asthma defined from an analysis of sputum proteomics. Although there has been evidence of complement activation in asthma, its role in severe asthma remains unclear.

methodSputum protein expression profiles were analyzed from healthy controls and severe asthma patients using data-dependent acquisition mass spectrometry. Weighted correlation network analysis (WGCNA) was used to define the unique modules that were highly correlated with clinical, physiologic, and inflammatory traits. Differential analysis was performed for the complement C5 pathway protein levels and eosinophilic protein expression as influenced by C5. Asthmatic mouse models were used to verify the effect of complement C5 administration and inhibition.

resultsThe WGCNA "brown" module related to the complement system activation was positively correlated with eosinophilic inflammation. Specifically, C5 and downstream complement proteins were up-regulated in patients with high sputum eosinophil levels (≥ 3%) compared to low sputum eosinophils (< 3%). Patients with reduced C5 expression had less eosinophilic inflammation and better lung function. Using single-cell RNA sequencing and immunofluorescence staining led to identification of macrophages as the main source of C5. In vivo experiments confirmed that inhibiting C5 reduced inflammation in allergic mouse models, while direct stimulation with recombinant C5 in IL-5 transgenic mice increased eosinophilic inflammation.

conclusionWe demonstrate a direct role for complement C5 in exacerbating eosinophilic inflammation in severe asthma.

Indexed as

AsthmaComplement C5EosinophilsInflammationAdultAnimalsComplement ActivationDisease Models, AnimalFemaleHumansMaleMiceMice, TransgenicMiddle AgedSeverity of Illness IndexSputumComplement C5C5aR1C5aR2complement C5complement C5 receptorseosinophilic inflammationsevere asthmaweighted correlation network analysis

Identifiers

PMID40524528
PMCPMC13139791

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.