Basal association of a transcription factor favors early gene expression.

Sandrine Pinheiro, Mariona Nadal-Ribelles, Carme Solé, Vincent Vincenzetti, Yves Dusserre, Francesc Posas, Serge Pelet

Abstract read

In one paragraph

Article in PLoS genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Kar4 acts as a Ste12 regulator inbioRxiv : the preprint server for biology · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sandrine PinheiroDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.

Mariona Nadal-RibellesDepartment of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.ORCID https://orcid.org/0000-0003-3314-6711

Carme SoléDepartment of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.ORCID https://orcid.org/0000-0003-4543-7401

Vincent VincenzettiDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.

Yves DusserreDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.

Francesc PosasDepartment of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.

Serge PeletDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.ORCID https://orcid.org/0000-0002-0245-049X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Responses to extracellular signals via Mitogen-Activated Protein Kinase (MAPK) pathways control complex transcriptional programs where hundreds of genes are induced at a desired level with a specific timing. Gene expression regulation is largely encoded in the promoter of the gene, which harbors numerous transcription factor binding sites. In the mating MAPK pathway of Saccharomyces cerevisiae, one major transcription factor, Ste12, controls the chronology of gene expression necessary for the fusion of two haploid cells. Because endogenous promoters encode a large diversity of Ste12 binding sites (PRE), we engineered synthetic promoters to decipher the rules that dictate mating gene induction. Conformations of PRE dimers that allow efficient gene expression were identified. The strength of binding of Ste12 to the PRE and the distance of the binding sites to the core promoter modulate the level of induction. The speed of activation is ensured by favoring a basal association of Ste12 by using a strong dimer of PRE located in a nucleosome depleted region.

Indexed as

Gene Expression Regulation, FungalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsBinding SitesMAP Kinase Signaling SystemMitogen-Activated Protein KinasesNucleosomesPromoter Regions, GeneticProtein BindingMitogen-Activated Protein KinasesNucleosomesSaccharomyces cerevisiae ProteinsSTE12 protein, S cerevisiaeTranscription Factors

Identifiers

PMID40523030

DOI10.1371/journal.pgen.1011710

PMCPMC12187013

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.