ArticleJournal of visualized experiments : JoVE2025
Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics.
Article in Journal of visualized experiments : JoVE, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- SSNA1 mechanically reinforces the damaged microtubule lattice.bioRxiv : the preprint server for biology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
In this protocol, we describe the design and fabrication of a microfluidic device developed for the investigation of microtubule polymer mechanics. The design utilizes the intrinsic benefits of Polydimethylsiloxane (PDMS)-based microfluidic devices and introduces several features to enable a robust and customizable high-throughput experimental approach. The developed device incorporates redundant bubble-trapping capabilities to prevent the occurrence of detrimental air bubbles. Furthermore, the device interfaces with an automated flow control system to reduce manual intervention and enable high-throughput analyses. Commercial simulation software is utilized to better develop and understand the fluid transport using this system. Finally, we demonstrate the capability to conduct multiple experiments simultaneously within a single device by growing microtubule extensions with distinct fluorescent labels in different sections of the device. Overall, this microfluidic flow system can be used to probe microtubule polymer mechanics and provides improvements in experimental design for broader microtubule in vitro studies. The synthesis of microfabrication, automated flow control, and computational modeling approaches enables a flexible system ideally suited for probing the cellular cytoskeleton in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.