Evidence map›Paper›PMID 40522008›Full record

ArticleJournal of cellular and molecular medicine2025

Adipocyte RNF20 Knockout Leads to Hyperinsulinemia via the H2Bub-H3K4me3-Slc2a4 Axis.

Ying Zhao, Xiaojuan Liang, Jiayu Tang, Chunwei Cao, Chunhuai Yang, Shulin Yang, Jianguo Zhao, Jinxiang Yuan, Meng Zhang, Yanfang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ying ZhaoState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Xiaojuan LiangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Jiayu TangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Chunwei CaoGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
Chunhuai YangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Shulin YangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Jianguo ZhaoState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Jinxiang YuanLin He's Academician Workstation of New Medicine and Clinical Translation, Jining Medical University, Jining, Shandong, China.
Meng ZhangCollege of Medical Imaging and Laboratory, Jining Medical University, Jining, Shandong, China.
Yanfang WangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.ORCID 0000-0003-1492-6771

Funding

Agricultural Science and Technology Innovation Program ASTIPAgricultural Science and Technology Innovation Program CAAS-SCAB-202301Key Program of National Natural Science Foundation of China 32230100Key Program of National Natural Science Foundation of China 32330099National Key R & D Program of China 2020YFA0509503National Natural Science Foundation for Distinguished Young Scholars 32025034Research Start-up Fund of Jining Medical University 600791001Shandong Provincial Higher Education Science and Technology Plan Project J18KA177
6 · The paper itself

Abstract

The ubiquitin ligase RING finger 20 (RNF20) mediated the monoubiquitination of histone H2B at lysine 120 (H2Bub), an epigenetic modification known to regulate key biological processes such as fat tissue development, tumorigenesis, spermatogenesis and so on. Despite our previous findings showing that mice with adipocyte-specific deletion of Rnf20 (ASKO mice) develop hyperinsulinaemia, the underlying mechanisms remain unclear. In this study, we investigated the role of adipocyte RNF20 in maintaining systemic insulin homoeostasis in ASKO mice. Our results reveal that ASKO mice exhibit an enlarged pancreas, increased islet size and a greater number of pancreatic β-cells. Fat tissue in ASKO mice showed reduced insulin sensitivity, evidenced by diminished AKT phosphorylation under basal and insulin-stimulated conditions, alongside suppressed insulin signalling pathways. Furthermore, the decreased levels of histone modifications, including H2Bub, H3K4me3 and H3K79me3, were observed in both ASKO mice fat tissues and Rnf20-knockdown 3T3-L1 cells. Mechanistically, Rnf20 knockdown in adipocytes reduced H3K4me3 occupancy at the Slc2a4 gene locus, inhibiting GLUT4 expression and inducing adipose-specific insulin resistance. These findings establish a critical role for adipocyte RNF20 in the insulin signalling regulation via the H2Bub-H3K4me3-Slc2a4 axis, highlighting its importance in systemic glucose metabolism.

Indexed as

AdipocytesGlucose Transporter Type 4HistonesHyperinsulinismUbiquitin-Protein Ligases3T3-L1 CellsAnimalsInsulinInsulin ResistanceInsulin-Secreting CellsMaleMiceMice, KnockoutSignal TransductionUbiquitinationGlucose Transporter Type 4histone H3 trimethyl Lys4HistonesInsulinRNF20 protein, mouseSlc2a4 protein, mouseUbiquitin-Protein Ligasesadipose tissueH3K4me3insulin resistanceRNF20Slc2a4

Identifiers

PMID40522008
PMCPMC12168218

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.