ArticleJournal of the American Society for Mass Spectrometry2025
Practical Tips for the Application of HDX-MS to Membrane Proteins, Biomolecular Condensates, and Weak Protein Binders.
Article in Journal of the American Society for Mass Spectrometry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Residue-Level Determination of Small-Molecule-Protein Affinities by Hydrogen-Deuterium Exchange Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) has emerged as a powerful biophysical tool to study protein thermodynamics, dynamics, and function. Despite its increasing use, effective implementation of HDX-MS requires knowledge of protein chemistry, folding, liquid chromatography, MS, and automation. Whereas method development for soluble globular proteins is often easily generalizable, HDX-MS on more complex systems is challenging. Published method sections are usually concise, and available step-by-step protocols typically only provide generalized procedures. Here, we offer tips for applying HDX-MS to three complex systems: integral membrane proteins, biomolecular condensates, and weak protein binders. These practices have yielded high sequence coverage and high-quality data, allowing for free energy quantification and, importantly, mechanistic understanding of protein functions. We believe that sharing our methodologies will help others apply and develop HDX-MS methods for these and similar systems.
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