Evidence map›Paper›PMID 40521560›Full record

ArticleACS omega2025

Setup of an In Vitro Three-Dimensional Stromalized Prostate Cancer Model Using Gelatin Microparticles.

Giulia Gangarossa, Marta Iozzo, Giulia Mugnaini, Rita Gelli, Luigi Ippolito, Elisa Giannoni, Giuseppina Comito, Massimo Bonini, Paola Chiarugi

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In one paragraph

Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Giulia GangarossaDepartment of Experimental and Clinical Biomedical Sciences, "Mario Serio", University of Florence, Viale Morgagni 50, 50134 Florence, Italy.ORCID https://orcid.org/0009-0008-3375-4577
Marta IozzoDepartment of Experimental and Clinical Biomedical Sciences, "Mario Serio", University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Giulia MugnainiDepartment of Chemistry "Ugo Schiff" and CSGI, University of Florence, Via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.
Rita GelliDepartment of Chemistry "Ugo Schiff" and CSGI, University of Florence, Via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.ORCID https://orcid.org/0000-0002-2917-574X
Luigi IppolitoDepartment of Experimental and Clinical Biomedical Sciences, "Mario Serio", University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Elisa GiannoniDepartment of Experimental and Clinical Biomedical Sciences, "Mario Serio", University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Giuseppina ComitoDepartment of Experimental and Clinical Biomedical Sciences, "Mario Serio", University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Massimo BoniniDepartment of Chemistry "Ugo Schiff" and CSGI, University of Florence, Via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.ORCID https://orcid.org/0000-0002-9041-6047
Paola ChiarugiDepartment of Experimental and Clinical Biomedical Sciences, "Mario Serio", University of Florence, Viale Morgagni 50, 50134 Florence, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Developing three-dimensional (3D) tumor models that accurately mimic the tumor microenvironment (TME) and its heterogeneity remains a significant challenge in preclinical research. Advancing these models holds the potential to improve the study of cancer pathologies in vitro, while reducing dependence on animal models. To tackle this challenge, in this work, we report on the development of an in vitro 3D stromalized prostate cancer model using gelatin porous microparticles as microscaffolds for cell attachment and growth. Gelatin porous microparticles were prepared by a double emulsion method and cross-linked with a biocompatible cross-linking agent, that is, glyceraldehyde, to prevent dissolution under physiological conditions. Then, we developed a stromalized 3D gelatin-based microscaffold biomimicking the interplay between human prostate cancer (PCa) and stromal cells by coculturing 22Rv1 cells and fibroblasts with gelatin porous microparticles. Overall, our results demonstrate the feasibility of gelatin microscaffolds in reproducing a 3D stromalized model of PCa progression (e.g., metabolic reprogramming), resulting from the tumor-stroma interaction. Thus, these systems represent a valuable platform and an effective tool for the study of cancer progression, such as TME biomimetics, while simultaneously offering a valid alternative to minimize the reliance on animal studies in preclinical research.

Identifiers

PMID40521560
PMCPMC12163836

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.