ArticleFrontiers in bioengineering and biotechnology2025
3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization.
Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Functional Group Chemistry Modulates Cellular Responses to Soluble Gelatin Derivatives Independent of Crosslinking.Biomolecules · 2026Article
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Abstract
The development of physiologically relevant three-dimensional (3D) culture platforms for neural stem cell (NSC) cultivation is essential for advancing neuroscience research, disease modelling, and regenerative medicine. In this study, we introduce norbornene-functionalized gelatin (GelNB) hydrogels crosslinked with a laminin-based peptide as a bioactive scaffold for NSC culture. A central composite design of experiments (DoE) approach was employed to systematically map hydrogel mechanical properties across varying macromer (4%-7%) and crosslinker (3-9 mM) concentrations via a response surface. This enabled precise tuning of hydrogel stiffness between 0.5 and 3.5 kPa, closely mimicking the mechanical properties of brain tissue. The optimized GelNB hydrogel formulation (5% GelNB, 8 mM crosslinker) supported NSC viability and enhanced NSC cluster formation demonstrating its suitability for 3D neural cell culture. Furthermore, we characterized the onset of hypoxia in 3D constructs using genetically encoded fluorescent hypoxia biosensors, revealing a cell density-dependent hypoxic response. At 3 × 10
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