Evidence map›Paper›PMID 40520966›Full record

ArticleOne health (Amsterdam, Netherlands)2025

Molecular detection and characterization of human bocavirus in environmental waters in Thailand from 2020 to 2022.

Kattareeya Kumthip, Pattara Khamrin, Arpaporn Yodmeeklin, Hiroshi Ushijima, Niwat Maneekarn

Abstract read
In one paragraph

Article in One health (Amsterdam, Netherlands), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kattareeya KumthipDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Pattara KhamrinDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Arpaporn YodmeeklinDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Hiroshi UshijimaDepartment of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Niwat ManeekarnDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human bocavirus (HBoV) is a globally recognized pathogen associated with respiratory and gastrointestinal infections in infants and young children. Its detection in both clinical and environmental water samples has raised concerns about potential waterborne transmission. This study investigated the prevalence and genetic diversity of HBoV in environmental water sources in Chang Mai, Thailand, from July 2020 to December 2022. A total of 600 water samples from various environmental sources were analyzed using PCR assay. Nucleotide sequencing and phylogenetic analysis were performed to identify HBoV genotypes and assess their genetic characteristics. HBoV was detected in 32 of 600 samples (5.3 %), representing a notable decline in the detection rates during the COVID-19 pandemic. Among the sample types, wastewater showed highest detection rate (23.3 %), followed by irrigation water (1.2 %), and river water (0.8 %). No HBoV was detected in environmental reservoir water samples. All four known HBoV genotypes (HBoV1-HBoV4) were identified, with HBoV2 being the most prevalent genotype (19 strains; 59.4 %), followed by HBoV3 (6 strains; 18.8 %), HBoV1 (5 strains; 15.6 %), and HBoV4 (2 strains; 6.2 %). No clear seasonal pattern of HBoV contamination was observed. Phylogenetic analysis revealed a close genetic relationship between environmental and human-associated HBoV strains, suggesting an interactive transmission of HBoV between human and the environment. This study highlights the prevalence and genetic diversity of HBoV in environmental waters during the COVID-19 pandemic and underscores the need for continued surveillance to a better understanding its environmental dynamics and potential public health implications.

Indexed as

Environmental waterGenotypeHuman bocavirusThailandWaterborne transmission

Identifiers

PMID40520966
PMCPMC12166461

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.