ArticleCureus2025
Effect of Demineralization Time on the Release of Bone Morphogenetic Protein in Indigenously Prepared Demineralized Freeze-Dried Bone Allografts: A Comparative In Vitro Study.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Partially demineralized allogeneic cancellous bone plug versus deproteinized bovine bone mineral for guided bone regeneration: a pilot randomized controlled clinical trial.Clinical oral investigations · 2026Trial
- The Therapeutic Scope of Orofacial Mesenchymal Stem Cells.Bioengineering (Basel, Switzerland) · 2025Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDemineralized freeze-dried bone allografts (DFDBAs) have emerged as a valuable biomaterial for regenerative therapy owing to their osteoinductive properties, which are attributable to the presence of bone morphogenetic proteins (BMPs). The different demineralization protocols used during graft processing by various tissue banks affect the levels of these growth factors. This study investigated the release of BMP-7 from a DFDBA prepared using two different demineralization time intervals.
methodA total of 144 bone graft samples were prepared using a standard protocol. The samples were divided into two groups, each containing 72. Group 1 samples were demineralized for 18 hours, and Group 2 samples were demineralized for 90 minutes. All samples were gamma-irradiated and subjected to the BMP-7 extraction procedure. Quantification of BMP-7 was done using enzyme-linked immunosorbent assay (ELISA).
resultsThe mean BMP-7 concentration of Group 1 was 135.609±11.943 ng/g and that of Group 2 was 95.442±9.226 ng/g. This difference between the mean BMP-7 concentration was found to be statistically significant (p = 0.013).
conclusionThe study evaluated the effect of demineralization time on BMP-7 levels in indigenously prepared DFDBAs. By unveiling the relationship between demineralization time and BMP content, this study paves the way for optimizing DFDBA quality and advancing its potential in regenerative therapy.
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