ArticleSmall (Weinheim an der Bergstrasse, Germany)2025
SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification.
Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Solvent-triggered reconfiguration of optical physical unclonable functions.Nature communications · 2026Article
- SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
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9 authors.
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Abstract
Studies on the dynamics of single cell phenotyping have been hampered by the lack of quantitative high-throughput metabolism assays. Extracellular acidification, a prominent phenotype, yields significant insights into cellular metabolism, including tumorigenicity. Here, it is developed a versatile microfluidic system for single cell optical pH analysis (SCO-pH), which compartmentalizes single cells in 140-pL droplets and immobilizes ≈40,000 droplets in a 2D array for temporal extracellular pH analysis. SCO-pH distinguishes cells undergoing hyperglycolysis induced by oligomycin A from untreated cells by monitoring their extracellular acidification. To facilitate pH sensing in each droplet, a cell-impermeable pH probe is encapsulated and its fluorescence intensities are quantified. Using this approach, hyperglycolytic cells can be differentiated, and single-cell heterogeneity in extracellular acidification dynamics can be concurrently observed. This high-throughput system will be useful in applications that require dynamic phenotyping of single cells with significant heterogeneity.
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