Safety evaluation and optimization of high-efficiency expression of 5hsCT in Saccharomyces cerevisiae.

Yakun Zhang, Wenlu Jia, Wenli Qiu, Yujing Tang, Hualin Wei, Hengyi Sun

Abstract read

In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yakun Zhang *School of Bioscience and Technology, Shandong Second Medical University, 7166 Baotong West Street, Weifang, 261053, Shandong, China.ORCID http://orcid.org/0009-0001-3863-3183

Wenlu Jia *School of Bioscience and Technology, Shandong Second Medical University, 7166 Baotong West Street, Weifang, 261053, Shandong, China.

Wenli QiuSchool of Bioscience and Technology, Shandong Second Medical University, 7166 Baotong West Street, Weifang, 261053, Shandong, China.

Yujing TangSchool of Bioscience and Technology, Shandong Second Medical University, 7166 Baotong West Street, Weifang, 261053, Shandong, China.

Hualin WeiSchool of Bioscience and Technology, Shandong Second Medical University, 7166 Baotong West Street, Weifang, 261053, Shandong, China.

Hengyi SunSchool of Bioscience and Technology, Shandong Second Medical University, 7166 Baotong West Street, Weifang, 261053, Shandong, China. sunhengyi1987@sdsmu.edu.cn.

Funding

Development Program for Youth Innovation Team in Colleges and Universities of Shandong Province 2022KJ263National Natural Science Foundation of China 82104053Natural Science Foundation of Shandong Province ZR2019BH035Natural Science Foundation of Shandong Province ZR2020MD094

6 · The paper itself

Abstract

To evaluate the oral safety of the industrial strain Saccharomyces cerevisiae YS-5hsCT (S. cerevisiae YS-5hsCT) expressing human/salmon chimeric calcitonin (5hsCT) and optimize its fermentation conditions, providing scientific basis for industrial production of oral recombinant calcitonin. The strain demonstrated an LD

Indexed as

CalcitoninRecombinant ProteinsSaccharomyces cerevisiaeAnimalsFermentationGene ExpressionHumansTemperatureCalcitoninRecombinant ProteinsChimeric human/salmon calcitoninExpressionOptimizationSaccharomyces cerevisiaeSafety evaluation

Identifiers

PMID40517205

DOI10.1016/j.ijpharm.2020.120171

PMCPMC12167324

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.