Evidence map›Paper›PMID 40516427›Full record

ArticleBiosensors & bioelectronics2025

Demonstration and structural basis of a therapeutic DNA aptamer for SARS-CoV-2 spike protein detection.

Yujun Liu, Kaidong Wang, Weiguang Wang, Saarang Kashyap, Jonathan Jih, Anthony Imani, Tzung Hsiai, Z Hong Zhou

Abstract read
In one paragraph

Article in Biosensors & bioelectronics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Yujun LiuCalifornia NanoSystems Institute, University of California at Los Angeles, Los Angeles, 90095, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, 90095, California, USA.
Kaidong WangDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA; Department of Medicine, Greater Los Angeles VA Healthcare System, Los Angeles, CA, 90073, USA; Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Weiguang WangCalifornia NanoSystems Institute, University of California at Los Angeles, Los Angeles, 90095, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, 90095, California, USA.
Saarang KashyapCalifornia NanoSystems Institute, University of California at Los Angeles, Los Angeles, 90095, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, 90095, California, USA.
Jonathan JihCalifornia NanoSystems Institute, University of California at Los Angeles, Los Angeles, 90095, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, 90095, California, USA; Molecular Biology Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, 90095, USA.
Anthony ImaniCalifornia NanoSystems Institute, University of California at Los Angeles, Los Angeles, 90095, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, 90095, California, USA.
Tzung HsiaiDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA; Department of Medicine, Greater Los Angeles VA Healthcare System, Los Angeles, CA, 90073, USA; Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA, 90095, USA. Electronic address: thsiai@mednet.ucla.edu.
Z Hong ZhouCalifornia NanoSystems Institute, University of California at Los Angeles, Los Angeles, 90095, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, 90095, California, USA; Molecular Biology Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, 90095, USA. Electronic address: Hong.Zhou@ucla.edu.

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
In situ atomic structures of the Kaposi's sarcoma-associated herpesvirus portal-terminase complex and glycoproteinsR01DE025567 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WU, TING-TING, ZHOU, Z HONG · 2016 to 2024
$4.0M
Shear Stress and Light-Sheets to Study Cardiac TrabeculationR01HL129727 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2015 to 2024
$3.5M
Electronica Impedance to Access Metabolically Active PlaqueR01HL118650 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K · 2014 to 2022
$3.3M
Intravascular Deployment of a Wirelessly Powered Micro-PacerR01HL149808 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Tzung K Hsiai · 2020 to 2026
$2.8M
Caltech/UCLA Individualized Theranostic Engineering to Advance Metabolic System (iTEAM)T32EB027629 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Dino Di Carlo, Azita Emami · 2020 to 2026
$2.0M
Integrating Volumetric Light-Field with Computational Fluid Dynamics to Study Myocardial Trabeculation and FunctionR01HL159970 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2021 to 2024
$2.0M
UCLA and Caltech integrated Cardiovascular Medicine for Bioengineers (iCMB)T32HL144449 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, PAJUKANTA, PAIVI · 2020 to 2024
$1.2M
Direct Detection Device for atomic resolution cryoEM of macromolecular complexesS10OD018111 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2014 to 2014
$598k
BLRD VA I01 BX004356CSRD VA I01 CX002705NHLBI NIH HHS R01 HL118650NHLBI NIH HHS R01 HL129727NHLBI NIH HHS R01 HL149808NHLBI NIH HHS R01 HL159970NHLBI NIH HHS T32 HL144449NIBIB NIH HHS T32 EB027629NIDCR NIH HHS R01 DE025567NIGMS NIH HHS R01 GM071940NIGMS NIH HHS R01 GM129325NIH HHS S10 OD018111
6 · The paper itself

Abstract

At the onset of the COVID-19 pandemic, the absence of rapid and precise diagnostic tools hindered early detection and response. To address this challenge, we developed a renewable electrochemical impedance biosensor (aptasensor) using a therapeutic DNA aptamer immobilized on a nanostructured gold nanoparticle/carbon nanotube (AuNP/CNT) electrode to detect the SARS-CoV-2 spike (S) protein receptor-binding domain (RBD). The aptasensor achieved a limit of detection of 0.19 pg mL

Indexed as

Aptamers, NucleotideBiosensing TechniquesCOVID-19SARS-CoV-2Spike Glycoprotein, CoronavirusDielectric SpectroscopyGoldHumansLimit of DetectionMetal NanoparticlesNanotubes, CarbonReproducibility of ResultsSurface Plasmon ResonanceAptamers, NucleotideGoldNanotubes, CarbonSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2AptamerCryoEMDetectionReceptor binding domainSARS-CoV-2Specificity

Identifiers

PMID40516427
PMCPMC12529732

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.