Protocol for laboratory rearing and infection tracking of Rhodnius prolixus using 3D-printable designs.

Ruby E Harrison, Ronald Drew Etheridge

Abstract read

In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ruby E HarrisonCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA. Electronic address: ruby.harrison25@uga.edu.

Ronald Drew EtheridgeCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA. Electronic address: ronald.etheridge@uga.edu.

Funding

Training in Tropical and Emerging Global Diseases - SupplementT32AI060546 · NIAID · UNIVERSITY OF GEORGIA (UGA) · PI Silvia N Moreno, Vasant Muralidharan · 2004 to 2026

$5.1M

Elucidating the Role of Endocytosis Via the Cytostome in the Life Cycle of Trypanosoma cruziR01AI163140 · NIAID · UNIVERSITY OF GEORGIA · PI ETHERIDGE, RONALD DREW · 2021 to 2025

$2.4M

Elucidating the Mechanistic Basis for Phagotrophy in the Protozoan Trypansoma cruziR01GM144545 · NIGMS · UNIVERSITY OF GEORGIA · PI ETHERIDGE, RONALD DREW · 2022 to 2025

$1.4M

NIAID NIH HHS R01 AI163140NIAID NIH HHS T32 AI060546NIGMS NIH HHS R01 GM144545

6 · The paper itself

Abstract

Human infections by Trypanosoma cruzi propagate via its blood-feeding triatomine vector. Investigating parasite-vector interactions depends upon robust techniques to rear insects and analyze infections. Here, we present a protocol for laboratory rearing and infection tracking of Rhodnius prolixus. We describe steps for housing, feeding, and sorting strategies using 3D-printable designs. We also detail procedures for gut dissection, fecal collection, and parasite re-isolation. This protocol describes techniques that support efforts to understand and mitigate vector-mediated Chagas disease transmission.

Indexed as

Insect VectorsPrinting, Three-DimensionalRhodniusTrypanosoma cruziAnimalsChagas DiseaseCell BiologyMicrobiologyProtocols in EntomologySpecial Issue

Identifiers

PMID40516057

DOI10.1016/j.xpro.2025.103894

PMCPMC12205801

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.