Protocol for production of homogeneous iPSC spheroids and microtissues using the suction technique.

Taijun Moriwaki, Hidenori Tani, Kotaro Haga, Shugo Tohyama

Abstract read

In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Human induced pluripotent stem cell-derived cardiomyocytes for disease modeling and drug discovery.Frontiers in bioengineering and biotechnology · 2025
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Taijun MoriwakiFujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan. Electronic address: taijun.moriwaki@fujita-hu.ac.jp.

Hidenori TaniFujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.

Kotaro HagaFujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan.

Shugo TohyamaFujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan; Keio University Regenerative Medicine Research Center, Kawasaki, Kanagawa, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan. Electronic address: shugo.tohyama@fujita-hu.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional cultures mimic in vivo environments better than two-dimensional cultures and are often used in drug discovery research. Herein, we present a protocol for producing homogeneous induced pluripotent stem cell (iPSC) spheroids and microtissues using the suction technique. We describe steps for preparing the suction device, preparing and seeding cells, and suction sedimentation of cells. We then detail procedures for self-assembly and evaluation of spheroids. For complete details on the use and execution of this protocol, please refer to Moriwaki et al.

Indexed as

Cell Culture TechniquesCell Culture Techniques, Three DimensionalInduced Pluripotent Stem CellsSpheroids, CellularHumansSuctionCell BiologyOrganoidsStem CellsTissue Engineering

Identifiers

PMID40516047

DOI10.1016/j.xpro.2025.103891

PMCPMC12207686

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.