ArticleMethods in molecular biology (Clifton, N.J.)2025
Activity-Based Protein Profiling to Study Virus-Host and Viral-Induced MicroRNA-Host Interactions.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
2 authors.
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Abstract
Activity-based protein profiling (ABPP) is a robust technique enabling the user to explore the functional implications of disease and pathology on key protein effectors. As such, a variety of activity-based probes have been developed and applied broadly to study virus-host interactions and the consequences of essential noncoding RNAs, like microRNAs, which viruses exploit for their propagation. The following protocol describes the process of infecting and transfecting cells for ABPP and covers the main ways in which ABPP can be used to study infection: in-gel fluorescence, western blotting, and mass spectrometry. Taken together, the findings of these experiments can be used to identify new therapeutic targets and to test novel antiviral inhibitors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.