Evidence map›Paper›PMID 40515988›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling.

Neetika Jaisinghani, Isabel Sakarin, Hiren V Patel, Michael Li, Mary L Previti, Jessica C Seeliger

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Neetika Jaisinghani *Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.
Isabel Sakarin *Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA.
Hiren V PatelDepartment of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.
Michael LiDepartment of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA.
Mary L PrevitiDepartment of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.
Jessica C SeeligerDepartment of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA. jessica.seeliger@stonybrook.edu.

Funding

Chemical Biology Training InterfaceT32GM136572 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI ELIZABETH M BOON, Jessica Chuang Seeliger · 2020 to 2026
$2.9M
Untangling the Inner Workings of the Mycobacterial Cell EnvelopeR35GM128552 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI SEELIGER, JESSICA CHUANG · 2018 to 2022
$2.1M
NIGMS NIH HHS R35 GM128552NIGMS NIH HHS T32 GM136572
6 · The paper itself

Abstract

Activity-based protein profiling (ABPP) enables the detection of protein reactivity across entire proteomes. Here, we describe the application of ABPP to the identification of serine hydrolase inhibitor targets in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis in humans. We highlight the adaptation of stable isotope labeling of amino acids (SILAC) to Mtb using a modified growth medium with an isotopically labeled sole nitrogen source and detail the resulting special requirements for proteomics analysis.

Indexed as

Bacterial ProteinsEnzyme InhibitorsMycobacterium tuberculosisProteomicsHumansIsotope LabelingProteomeBacterial ProteinsEnzyme InhibitorsProteomeActivity-based protein profilingInhibitorMycobacteriaProteomicsSILACTuberculosis

Identifiers

PMID40515988

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.