ArticleMetabolomics : Official journal of the Metabolomic Society2025
Untargeted analysis of hydrophilic metabolites using enhanced LC-MS separation with a pentafluoro phenylpropyl-functionalized column and prediction-based MS/MS spectrum annotation.
Article in Metabolomics : Official journal of the Metabolomic Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
introductionWide-targeted metabolome analysis has been applied in studies on the biology of mammals, plants, and microorganisms. However, there are still issues regarding both analytical and informatics technologies for establishing an untargeted and comprehensive analysis of hydrophilic primary and secondary metabolites.
objectivesThis study aimed to develop an improved chromatographic method for analyzing hydrophilic metabolites and an annotation method for these diverse metabolites.
methodsWe investigated the performance of a pentafluoro phenylpropyl-functionalized column (PFP column) for the comprehensive analysis of hydrophilic metabolites by liquid chromatography-mass spectrometry (LC-MS). Peaks were annotated using MS/MS spectral similarity searches of the predicted and experimental MS/MS spectra in metabolite structure databases.
resultsThe improved retention and peak shapes of the standard compounds were obtained using LC-MS analysis with a PFP column. The mobile phases comprised water with 0.1% formic acid and methanol with 0.1% formic acid and 10 mM ammonium formate. From the annotation results of the 48 standard compounds, the chemical structures were correctly annotated in 54% of the compounds. However, over 70% of the compounds were annotated as biologically relevant based on the natural product classification. When these methods were applied to the analysis of tomato fruits, 658 and 458 peaks were detected and annotated in the positive and negative ion analyses, respectively.
conclusionMetabolome analysis combined with LC-MS analysis and annotation can contribute to the comprehensive analysis of hydrophilic metabolites.
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