Evidence map›Paper›PMID 40515927›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Single-Particle Cryo-EM to Investigate Nonenveloped Virus-Like Particles.

Ping Chen, Chunfu Zheng, Zheng Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ping ChenCenter for Teaching and Learning Development, Southern Medical University, Guangzhou, Guangdong, China.
Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Zheng LiuKobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, China. liuzheng@cuhk.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nonenveloped viruses own numerous significant members that cause substantial agricultural losses and impact human health. Structural studies on these viruses are essential for understanding their mechanisms of pathogenesis and infection. Here, we describe protocols for determining high-resolution structures of nonenveloped viruses via cryogenic electron microscopy (cryo-EM). The steps include virus-like particle purification, cryo-grid preparation, cryo-EM image acquisition, and image processing for 3D reconstruction. These protocols can generally be applied to structural studies of all other nonenveloped viruses.

Indexed as

Cryoelectron MicroscopyVirionHumansImage Processing, Computer-AssistedImaging, Three-DimensionalCryo-electron microscopyNonenveloped virusSingle-particle analysisStructure determination

Identifiers

PMID40515927

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.