Evidence map›Paper›PMID 40515920›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2025

Isotope Labeling Techniques for Virus-Infected Cells.

Jiayuan Wu, Yuantao Zhou, Yumiao Liu, Chunfu Zheng, Li Li

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jiayuan WuKunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Institute of Pediatrics, Yunnan Province Clinical Research Center for Children's Health and Disease, Kunming Children's Hospital, Kunming, Yunnan, China.
Yuantao ZhouKunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Institute of Pediatrics, Yunnan Province Clinical Research Center for Children's Health and Disease, Kunming Children's Hospital, Kunming, Yunnan, China.
Yumiao LiuKunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Institute of Pediatrics, Yunnan Province Clinical Research Center for Children's Health and Disease, Kunming Children's Hospital, Kunming, Yunnan, China.
Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Li LiKunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Institute of Pediatrics, Yunnan Province Clinical Research Center for Children's Health and Disease, Kunming Children's Hospital, Kunming, Yunnan, China. lili10@kmmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The viral infection of host cells is a dynamic and complex process characterized by multifaceted interactions and metabolic shifts between the virus and host. In order to better understand the molecular mechanisms involved in the viral life cycle-particularly replication, assembly, release, and the host cell response-researchers have turned to isotope labeling techniques. The employment of isotope labeling techniques as a precise method to track molecular changes is a common practice in the research community, with the aim of gaining deeper insights into the molecular mechanisms involved in the viral life cycle, including replication, assembly, release, and the host cell response. This review aims to provide a comprehensive summary of the current isotope labeling techniques commonly used for the analysis of virus-infected cells. It will also outline the relevant research protocols and their advantages and limitations. When employed in conjunction with high-resolution mass spectrometry, isotope labeling provides enhanced quantitative data, allowing researchers to track molecular dynamics in viruses and host cells with a high degree of precision.

Indexed as

Isotope LabelingVirus DiseasesVirusesAnimalsHost-Pathogen InteractionsHumansMass SpectrometryVirus ReplicationHostIsotope labelingMetabolomicsMetabolomicsProteomicsViral infectionVirus interactions

Identifiers

PMID40515920

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.