Chromatin Immunoprecipitation (ChIP)-Quantitative PCR (qPCR) Analysis of Viral Genome-Host Protein Interactions.

Zhe Wang, Penghui Hu, Zemin Ji, Xiao Shan, Chunfu Zheng, Qiujing Yu

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Zhe WangDepartment of Health Management Center & Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

Penghui HuDepartment of Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin, China.

Zemin JiDepartment of Health Management Center & Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

Xiao ShanDepartment of Health Management Center & Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.

Qiujing YuDepartment of Health Management Center & Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China. yuqiujing@uestc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein-DNA interactions play crucial roles in various biological processes, such as transcriptional regulation, histone modifications, DNA damage repair, and cell cycle progression. Chromatin immunoprecipitation (ChIP) is a powerful technique used to study interactions between proteins and DNA within cells. This technique has not only clarified the relationships among transcription factors, histone modifications, and gene regulation but also facilitated extensive genome-wide analyses of protein-DNA interactions. In this chapter, we provide a detailed overview of the ChIP-quantitative PCR (qPCR) technique, emphasizing its application in investigating the interactions between viral genomes and host proteins through ChIP-qPCR analysis.

Indexed as

Chromatin ImmunoprecipitationGenome, ViralHost-Pathogen InteractionsReal-Time Polymerase Chain ReactionDNA, ViralHumansProtein BindingDNA, ViralChIPChIP-qPCRHost proteinsHost proteinsProtein-DNA interactionViral genomes

Identifiers

PMID40515916

DOI10.1038/nrg2641

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.