Evidence map›Paper›PMID 40515913›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Engineering of the WSN Strain for Investigating Antiviral and Antitumor Immunity.

Yu Han, Yi Yuan, Feng Ma

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yu Han *National Key Laboratory of Immunity and Inflammation, and CAMS Key Laboratory of Synthetic Biology Regulatory Elements, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China.
Yi Yuan *National Key Laboratory of Immunity and Inflammation, and CAMS Key Laboratory of Synthetic Biology Regulatory Elements, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China.
Feng MaNational Key Laboratory of Immunity and Inflammation, and CAMS Key Laboratory of Synthetic Biology Regulatory Elements, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China. maf@ism.pumc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Influenza viruses are highly variable and transmissible, often causing seasonal influenza outbreaks worldwide. The genomic sequence, protein structure and function, and the viral packaging mechanism have been extensively studied and well characterized, positioning influenza viruses as key models for the identification and validation of conditionally controlled genetic elements. With the development of reverse genetics and synthetic biology, genetically engineered influenza viruses can more effectively control virus replication, thereby increasing vaccine safety and inducing a robust immune response in the host. In addition, engineered influenza viruses have garnered extensive attention in the field of tumor immunotherapy. Here, we describe methods for genetically engineering the H1N1 strain WSN on the basis of the influenza A virus reverse genetic system and provide detailed insights and perspectives on their applications.

Indexed as

Genetic EngineeringInfluenza A Virus, H1N1 SubtypeNeoplasmsAnimalsHumansReverse GeneticsVirus ReplicationAntitumor immunityAntiviral immunityInfluenza virusReverse genetic systemWSN

Identifiers

PMID40515913

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.