Evidence map›Paper›PMID 40515906›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.

Sisi Xia, Jialin Wang, Xiaoping Huo, Chunfu Zheng, Hongjian Li, Jun Chen

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sisi XiaDepartment of Biological Engineering, Wuhan Polytechnic University, Wuhan, China.
Jialin WangKey Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou, China.
Xiaoping HuoKey Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou, China.
Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Hongjian LiState Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, China. tlihj@jnu.edu.cn.
Jun ChenState Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, China. chenjun@jnu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-throughput yeast two-hybrid (YTH) screening systems are essential technologies for understanding the viral life cycle, pathogenesis, and development of antiviral therapies. They are commonly used in molecular virology to identify and analyze the complex molecular interactions between viruses and host cells. This chapter details the relevant information and experimental procedures for systematically screening and identifying interactions between viral and host proteins via high-throughput YTH technology.

Indexed as

High-Throughput Screening AssaysTwo-Hybrid System TechniquesVirologyVirusesHost-Pathogen InteractionsHumansSaccharomyces cerevisiaeViral ProteinsViral ProteinsHigh-throughput YTH technologyHost cellsMolecular interactionsMolecular virologyViruses

Identifiers

PMID40515906

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.