Evidence map›Paper›PMID 40515902›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

In Vitro Liquid-Liquid Phase Separation Assay for Viral Proteins.

Meilin Hu, Xiaoqing Liu, Taizhen Liang, Chunfu Zheng, Xiancai Ma

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Meilin HuGuangzhou National Laboratory, Guangzhou International Bio-Island, Guangzhou, China.
Xiaoqing LiuGuangzhou National Laboratory, Guangzhou International Bio-Island, Guangzhou, China.
Taizhen LiangGuangzhou National Laboratory, Guangzhou International Bio-Island, Guangzhou, China.
Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Xiancai MaGuangzhou National Laboratory, Guangzhou International Bio-Island, Guangzhou, China. ma_xiancai@gzlab.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In recent years, numerous membraneless condensates have been found to regulate cellular and viral processes. These condensates are mainly composed of specific proteins with liquid-liquid phase separation (LLPS) properties. Many studies have revealed that these membraneless organelles efficiently promote viral infection, replication, assembly, and latency, demonstrating their druggable potential by targeting LLPS. In vitro LLPS assays have been widely used to characterize the biophysical properties and study the biochemical functions of phase-separated proteins. By simulating the intracellular environment, these assays can elucidate how cellular or viral proteins form dynamically reversible condensates via LLPS. Here, we describe the procedure of the in vitro LLPS assay for viral proteins, which can also be applied to study the LLPS properties of cellular proteins.

Indexed as

Liquid-Liquid ExtractionViral ProteinsBiomolecular CondensatesHumansPhase SeparationViral ProteinsIntrinsically disordered proteinLiquid–liquid phase separationMicroscopyProtein concentrationProtein purificationSize-exclusion chromatography

Identifiers

PMID40515902

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.