ArticleMicrobial biotechnology2025
Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating.
Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Article
- Alkali Source Modulates Polydopamine Shell Thickness and Ferroptosis Susceptibility in Iron Oxide Nanoparticles Under Magnetic Hyperthermia.Bioinorganic chemistry and applications · 2026Article
- Scalable recombinant production of bioactive human neutrophil peptide-1 in Komagataella phaffii.AMB Express · 2025Article
- Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating.Microbial biotechnology · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
Komagataella phaffii is among the most widely used expression systems, with methanol-inducible promoters being preferred for protein expression due to their stringent regulation and exceptional strength. However, the applicability of this system, particularly in food and pharmaceutical products, is limited by methanol's toxic and pro-inflammatory properties. Therefore, obtaining a novel methanol-free expression system is necessary. In this study, we obtained a novel expression plasmid, pHSPαA, carrying the HSP70 promoter (P
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