Evidence map›Paper›PMID 40515400›Full record

ArticlePlant communications2025

OsBRK1-mediated phosphorylation of OsPFN2 regulates meiotic spindle-actin assembly and rice fertility.

Hai Zheng, Zhigang Zhao, Shanshan Zhu, Yulong Ren, Jiangfeng Shen, Ziqi Xun, Xiaowen Yu, Chaolong Wang, Bowen Yao, Siqi Cheng and 22 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors.

Hai ZhengState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Zhigang ZhaoState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Shanshan ZhuState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Yulong RenState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Jiangfeng ShenCenter for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100081, China.
Ziqi XunState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xiaowen YuState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Chaolong WangState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Bowen YaoState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Siqi ChengState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Yang HuState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Shihao ZhangState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Qiming WangState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Jiayu LuState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Zhenwei XieState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Dekun LeiState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Anqi JianState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Minrui ChenState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Keyi ChenState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Shijia LiuState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Xi LiuState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Yunlu TianState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Lin JiangState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Zhijun ChengState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Cailin LeiState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Qibing LinState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xiupin GuoState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xin WangState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Chuanyin WuState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Haiyang WangState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Shanjin HuangCenter for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100081, China. Electronic address: sjhuang@tsinghua.edu.cn.
Jianmin WanState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wanjianmin@caas.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Formation of a meiotic spindle structure is crucial for chromosome segregation and fertility in plants. Previous studies have shown that actin decorates spindle microtubules in mammalian oocytes, forming spindle actin, which is indispensable for genome stability and gamete segregation. However, the regulatory mechanisms that underlie spindle-actin assembly remain unknown. Here, we report that dysfunction of OsPFN2, a rice profilin protein, disrupts meiotic spindle-actin assembly and spindle microtubule structure and causes errors in chromosome alignment and segregation in pollen mother cells, resulting in male sterility. Furthermore, our results demonstrate that OsPFN2 interacts with Rice Morphology Determinant (OsRMD), a rice formin protein whose depletion also affects spindle-actin assembly and the structure of meiotic spindle microtubules. Intriguingly, we identified an interaction between OsPFN2 and Bub1-Related Kinase 1 (OsBRK1) and demonstrated that OsBRK1 depletion enhances spindle-actin assembly. In addition, we found that OsBRK1 phosphorylates OsPFN2 and that the resulting phosphorylated OsPFN2 retains its capability to bind actin. However, these phospho-mimetic actin-OsPFN2 complexes are not used by OsRMD. Our findings thus reveal that the OsPFN2-OsRMD module controls the assembly of meiotic spindle actin and that OsBRK1 fine-tunes this process through phosphorylation of OsPFN2.

Indexed as

ActinsOryzaPlant ProteinsProfilinsProtein Serine-Threonine KinasesSpindle ApparatusMeiosisMicrotubulesPhosphorylationPlant InfertilityActinsPlant ProteinsProfilinsProtein Serine-Threonine Kinasesmale sterilityOryza sativapollen developmentricespindle-actin assemblyspindle morphogenesis

Identifiers

PMID40515400
PMCPMC12365840

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.