Evidence map›Paper›PMID 40513074›Full record

ArticleJournal of the American Chemical Society2025

Designing Reversible Photoswitching Azobenzene-Modified Nucleotide for Controlling Biological Function.

Juncheng Li, Jinxi Du, Weiwei He, Ibrahim O Adelakun, Miao Zhong, Savia Boyer, Ya Ying Zheng, Qishan Lin, Serdal Kirmizialtin, Jia Sheng and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Light-responsive DNA nanostructures.Nature reviews. Chemistry · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Juncheng LiThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
Jinxi DuThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.ORCID 0009-0004-8865-2210
Weiwei HeChemistry Program, Science Division, New York University, Abu Dhabi 129188, United Arab Emirates.
Ibrahim O AdelakunThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
Miao ZhongThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
Savia BoyerThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
Ya Ying ZhengThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
Qishan LinThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
Serdal KirmizialtinChemistry Program, Science Division, New York University, Abu Dhabi 129188, United Arab Emirates.ORCID 0000-0001-8380-5725
Jia ShengThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.ORCID 0000-0001-6198-390X
Ting WangThe RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.ORCID 0000-0001-7630-4148

Funding

Optimizing Enhanced Hammerhead Ribozymes for Retinal Nucleic Acid TherapeuticsR01EY035007 · NEI · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI JOHN M. SULLIVAN · 2023 to 2026
$2.2M
Chemical Modifications to Wobble Uridines in tRNA Regulate Responses to StressR01GM143749 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI BEGLEY, THOMAS J, SHENG, JIA · 2022 to 2025
$1.4M
NEI NIH HHS R01 EY035007NIGMS NIH HHS R01 GM143749
6 · The paper itself

Abstract

RNA plays vital roles in numerous normal and diseased cellular functions and processes. Reversible photoregulation of oligonucleotide's structure and function is a powerful strategy for both regulating biological processes and developing novel RNA-based therapeutics. Herein, we designed an azobenzene-modified cytidine phosphoramidite and synthesized a series of oligoribonucleotides containing this photoswitchable residue. We validated the reversible photoisomerization in both ribonucleoside and oligoribonucleotide contexts and studied the overall impact of this cytidine modification through all-atom molecular dynamics (MD) simulations and UV melting experiments. We also showed that the modified oligoribonucleotide can switch the reverse transcription (RT) process upon light irradiation in the presence of various RT enzymes. In addition, the optical control mechanism of the HIV reverse transcriptase-mediated RT process was elucidated by MD simulation. This new chemical biology toolset enables reversible optical control of RNA structures and functions for gene regulation and novel drug development.

Indexed as

Azo CompoundsNucleotidesHIV Reverse TranscriptaseMolecular Dynamics SimulationOrganophosphorus CompoundsPhotochemical ProcessesUltraviolet RaysazobenzeneAzo CompoundsHIV Reverse TranscriptaseNucleotidesOrganophosphorus Compoundsphosphoramidite

Identifiers

PMID40513074
PMCPMC12203576

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.