Quantification of Tick-Borne Flaviviruses Using Focus Forming Assays.

Allen T Esterly, Erin S Reynolds, Saravanan Thangamani

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Defining the kinetics of severe fever with thrombocytopenia syndrome virus acquisition and dissemination in naturally-infectedFrontiers in cellular and infection microbiology · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Allen T EsterlySection of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA.

Erin S ReynoldsDepartment of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY, USA.

Saravanan ThangamaniSUNY Center for Vector-Borne Diseases, SUNY Upstate Medical University, Syracuse, NY, USA. thangams@upstate.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tick-borne flaviviruses, such as tick-borne encephalitis virus (TBEV), Omsk hemorrhagic fever virus (OHFV), and Powassan virus (POWV), pose significant threats to human health.POWV is an emerging arbovirus and is the only member of the tick-borne encephalitis serogroup causing public health concern in North America, specifically in the Northeastern United States and Eastern Canada (Hermance and Thangamani, Vector Borne Zoonotic Dis 17(7):453-462, 2017; Hassett and Thangamani, Microorganisms 9(11):2317, 2021). POWV lineages currently described, POWV lineage I, referred to as POWV, and POWV lineage II, referred to as Deer tick virus (DTV), are genetically similar and cause similar neurotropic disease in humans during spillover infections. Accurate quantification of POWV or DTV is critical to studying the basic biology, pathogenesis, and transmission physiology of these viruses. Herein, we describe our process to quantify POWV or DTV in a Biosafety Level 3 (BSL-3) setting using a repeatable methodology.

Indexed as

Encephalitis, Tick-BorneEncephalitis Viruses, Tick-BorneFlavivirusAnimalsHumansTicksDeer tick virusPowassan virusTick-borne diseaseTick-borne virusViral quantification

Identifiers

PMID40512314

DOI10.3390/microorganisms9112317

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.