Evidence map›Paper›PMID 40512309›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection.

Stephanie Beane, Megan Burch, Jeremy Bechelli

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Stephanie BeaneDepartment of Biological Sciences, Sam Houston State University, Huntsville, TX, USA.
Megan BurchDepartment of Biological Sciences, Sam Houston State University, Huntsville, TX, USA.
Jeremy BechelliDepartment of Biological Sciences, Sam Houston State University, Huntsville, TX, USA. Jbechelli@salemstate.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorado tick fever virus (CTFV) is a double-stranded RNA virus in the family Reoviridae. CTFV is the causative agent of Colorado tick fever (CTF), a febrile illness that can have mild to severe manifestations. CTFV is an underreported and underrecognized virus; similarly, the literature concerning neglected tick-borne viruses lacks standard protocols for viral research. Here we describe protocols and procedures for CTFV studies, including the quantification of the virus through viral plaque assay, generation and storage of CTFV stocks, and detection with reverse transcription-quantitative PCR (RT-qPCR).

Indexed as

Colorado Tick FeverColorado tick fever virusVirus CultivationAnimalsHumansReal-Time Polymerase Chain ReactionRNA, ViralViral Plaque AssayRNA, ViralCell cultureColorado tick feverColorado tick fever virusTick-borne diseaseVero cellsViral detectionViral propagationViral quantification

Identifiers

PMID40512309

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.