Evidence map›Paper›PMID 40512258›Full record

ArticleMikrochimica acta2025

A robust DNA walker for simplified, rapid, and sensitive detection of HBV DNA in blood.

Rongji Lai, Mingshuang Lai, Baoren He, Bin Li, Linbin Huang, He Xie, Limin Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rongji Lai *The Joint Laboratory On Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Mingshuang Lai *The Joint Laboratory On Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Baoren HeThe Joint Laboratory On Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Bin LiThe Joint Laboratory On Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Linbin HuangThe Joint Laboratory On Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
He XieXidian Group Hospital, Xian, China.
Limin ChenThe Joint Laboratory On Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China. limin_chen_99@126.com.

Funding

Guangxi Natural Science Foundation Youth Science Foundation, China NO. 2024GXNSFBA010047Medical research project of Xi'an Science and Technology Bureau 22YXYJ0120Qin Chuangyuan recruited high-level innovation and entrepreneurship talents project of Science and Technology Department of Shaanxi Province QCYRCXM-2022-56vertical research project of General Universal Medical Group UM0223002, UM0223003
6 · The paper itself

Abstract

Although preventive vaccine is available, HBV infection continues to be a global health challenge, especially in rapid and accurate screening in resource-limited regions due to underdeveloped infrastructures. In this study, we developed a self-powered DNA walker system integrating AuNPs and DNAzyme for direct detection of HBV DNA in blood. In the presence of HBV DNA, this DNA walker was activated to start autonomous and rapid movement on AuNPs driven by DNAzyme, and the amplified fluorescent signals were generated and detected. With remarkable specificity and biostability, this DNA walker system was validated by detecting HBV DNA in blood samples from patients chronically infected with HBV. Our results demonstrated that the HBV DNA load of 20,000 IU/mL in plasma could be detected by our strategy, and there was a strong correlation (Pearson's r = -0.86) between the fluorescence intensity measured by the DNA walker and the Ct value. Collectively, we established a simple, efficient, and rapid detection strategy for HBV DNA in the blood, with substantial potential application in resource-limited countries/regions.

Indexed as

Biosensing TechniquesDNA, ViralHepatitis B virusDNA, CatalyticHumansLimit of DetectionDNA, CatalyticDNA, ViralDNA walkerDNAzymeFluorescence detectionHBV DNA detection

Identifiers

PMID40512258

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.