Evidence map›Paper›PMID 40512079›Full record

ArticlePediatric blood & cancer2025

PD-L1 Expression is Mediated by microRNA Processing, Wnt/β-Catenin Signaling, and Chemotherapy in Wilms Tumor.

Patricia D B Tiburcio, Kavita Desai, Austin Warne, Arash Nabbi, Serena Zhou, Sean D Reiff, Matthew E Campbell, James F Amatruda, Kenneth S Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pediatric blood & cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Patricia D B TiburcioDepartment of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Kavita DesaiUniversity of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA.
Austin WarneDepartment of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Arash NabbiPrincess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.
Serena ZhouDepartment of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.ORCID https://orcid.org/0009-0004-2846-7566
Sean D ReiffDepartment of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Matthew E CampbellDepartment of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
James F AmatrudaCancer and Blood Disease Institute, Children's Hospital Los Angeles, Los Angeles, California, USA.
Kenneth S ChenDepartment of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.ORCID https://orcid.org/0000-0003-2304-4631

Funding

UT Southwestern Medical Center Simmons Comprehensive Cancer CenterP30CA142543 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Kathryn Ann O'Donnell · 2010 to 2026
$53.7M
University of Texas Southwestern Medical Center SPORE in Kidney CancerP50CA196516 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Payal Kapur, Payal Kapur · 2016 to 2026
$24.7M
Developmental transcription factors in Drosha-driven cancerR01CA289259 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Kenneth Sung-Man Chen · 2024 to 2026
$2.2M
The role of PLAG1 in Wilms tumor formationK08CA207849 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI CHEN, KENNETH SUNG-MAN · 2017 to 2021
$781k
Alex's Lemonade Stand FoundationCancer Prevention and Research Institute of Texas RR180071NCI NIH HHS K08 CA207849NCI NIH HHS K08CA207849NCI NIH HHS P30 CA142543NCI NIH HHS P30CA142543NCI NIH HHS P50 CA196516NCI NIH HHS P50CA196516NCI NIH HHS R01CA289259Pablove FoundationU.S. Department of Defense KC220019
6 · The paper itself

Abstract

backgroundInhibition of immune checkpoint proteins is effective in adult cancers but has shown limited efficacy in pediatric cancers. While factors regulating expression of immune checkpoint proteins such as PD-L1 are well documented in adult cancers, their regulation is poorly understood in pediatric cancers.

methodsWe analyzed Wilms tumor specimens with reverse-phase protein arrays. We validated correlations using published sequencing data, flow cytometry, and immunoblots.

resultsUsing unsupervised clustering of protein arrays, we found that immune markers like PD-L1 are upregulated in distinct subsets of Wilms tumor, the most common pediatric kidney cancer. Specifically, chemotherapy-exposed Wilms tumor specimens exhibited higher levels of PD-L1 expression, and common chemotherapeutics upregulated PD-L1 in vitro. Furthermore, mutations in CTNNB1 and DROSHA, the two most commonly mutated genes in Wilms tumor, correlated with higher PD-L1. Activation of Wnt/β-catenin signaling and knockdown of DROSHA or DICER1 both increase PD-L1 in vitro.

conclusionsTogether, our results identify clinical and biological properties regulating PD-L1 in Wilms tumor that may inform precision therapy approaches in pediatric immuno-oncology.

Indexed as

B7-H1 AntigenGene Expression Regulation, NeoplasticKidney NeoplasmsMicroRNAsWilms TumorWnt Signaling Pathwaybeta CateninBiomarkers, TumorChildChild, PreschoolFemaleHumansMaleMutationRibonuclease IIIB7-H1 Antigenbeta CateninBiomarkers, TumorCD274 protein, humanCTNNB1 protein, humanDROSHA protein, humanMicroRNAsRibonuclease IIIimmunotherapymolecular geneticswilms tumor

Identifiers

PMID40512079
PMCPMC13231507

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.