Evidence map›Paper›PMID 40511924›Full record

ArticleJournal of clinical microbiology2025

Accurate and reproducible whole-genome genotyping for bacterial genomic surveillance with Nanopore sequencing data.

K Prior, K Becker, C Brandt, A Cabal Rosel, J Dabernig-Heinz, C Kohler, M Lohde, W Ruppitsch, F Schuler, G E Wagner and 1 more

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Nanopore Sequencing for Chikungunya Virus: Principles and Application.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Review
  3. Applicability of Nanopore-only whole-genome sequencing forJournal of clinical microbiology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. The draft genome sequence ofMicrobiology resource announcements · 2026
    Article
  9. Draft genome sequence ofMicrobiology resource announcements · 2026
    Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Complete genome ofMicrobiology resource announcements · 2025
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

K PriorDepartment of Periodontology and Operative Dentistry, University Hospital Münster, Münster, Germany.
K BeckerFriedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
C BrandtInstitute for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany.
A Cabal RoselInstitute for Surveillance and Infectious Disease Epidemiology, Austrian Agency for Health and Food Safety, Vienna, Austria.
J Dabernig-HeinzDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.
C KohlerFriedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
M LohdeInstitute for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany.
W RuppitschInstitute for Surveillance and Infectious Disease Epidemiology, Austrian Agency for Health and Food Safety, Vienna, Austria.ORCID 0000-0001-9940-3333
F SchulerInstitute of Medical Microbiology, University Hospital Münster, Münster, Germany.
G E WagnerDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.
A MellmannInstitute of Hygiene, University Hospital Münster, Münster, Germany.ORCID 0000-0002-0649-5185

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite recent advances in error rate reduction, until recently, Oxford Nanopore Technologies (ONT) sequences lacked the accuracy required for fine-scale bacterial genomic analysis. Here, recent software improvements of ONT and the ONT-core-genome multilocus sequence typing (cgMLST)-Polisher within the SeqSphere

Indexed as

BacteriaGenome, BacterialGenotyping TechniquesNanopore SequencingWhole Genome SequencingDrug Resistance, Multiple, BacterialGenomicsGenotypeHigh-Throughput Nucleotide SequencingHumansMultilocus Sequence TypingReproducibility of ResultsSoftwareerror rateNanopore sequencingpolishingring trial

Identifiers

PMID40511924
PMCPMC12239720

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.