Evidence map›Paper›PMID 40511256›Full record

ArticleRegenerative therapy2025

Platelet-rich fibrin-conditioned medium promotes osteogenesis of dental pulp stem cells through TGF-β and PDGF signaling.

Yasuyuki Fujii, Takashi Yoshida, Ayaka Sato, Mikiko Ikehata, Ayano Hatori, Daichi Chikazu, Shahram Ghanaati, Yoko Kawase-Koga

Abstract read
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In one paragraph

Article in Regenerative therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yasuyuki FujiiDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Takashi YoshidaDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Ayaka SatoDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Mikiko IkehataDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Ayano HatoriDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Daichi ChikazuDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Shahram GhanaatiFORM, Frankfurt Oral Regenerative Medicine, Clinic for Maxillofacial and Plastic Surgery, Goethe University, Theodor-Stern-Kai 7, 60596 Frankfurt, Germany.
Yoko Kawase-KogaDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Mesenchymal stem cells (MSCs), a key cell source for regenerative medicine, are conventionally cultured in human autologous serum (HAS) or fetal bovine serum (FBS). Platelet-rich fibrin-conditioned medium (PRF-CM) has emerged as a potential alternative to FBS, promoting the osteogenic differentiation of dental pulp stem cells (DPSCs) more efficiently than FBS. However, the molecular mechanisms underlying PRF-CM-induced osteogenesis in DPSCs remain unclear. Therefore, the aim of this study was to elucidate these mechanisms. Methods: PRF-CM was prepared from peripheral blood samples collected from three healthy donors. DPSCs were derived from the dental pulp extracted from the third molars of three healthy donors. The mRNA expression patterns of DPSCs cultured in FBS or PRF-CM were compared using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) were analyzed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Results: The mean blood volume collected from the three donors was 51.3 mL, yielding a mean PRF-CM volume of 168 mL. RNA-Seq identified 2258 DEGs, and KEGG pathway and GO analysis revealed that PRF-CM upregulated biological activities associated with transforming growth factor beta (TGF-β) signaling and platelet-derived growth factor (PDGF) binding compared with FBS. Western blotting analysis revealed that pSMAD2/3 and phosphor-Akt ( Conclusions: PRF-CM activates TGF-β and PDGF signaling pathways, thereby promoting the osteogenic differentiation of DPSCs. Moreover, the volume of PRF-CM obtained was approximately three times greater than the blood sample volume. These findings suggest that PRF-CM could serve as an effective alternative to HAS or FBS for bone regeneration therapy using MSCs.

Indexed as

Bone regenerationDental pulp stem cellsOsteogenic differntiationPlatelet-rich fibrin

Identifiers

PMID40511256
PMCPMC12159295

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.