ArticlemicroPublication biology2025
Extracellular stimulation in neocortex lacks specificity.
Article in microPublication biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
4 authors.
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Abstract
By activating axons, extracellular stimulation elicits synaptic responses in patch-clamped cells. However, because axonal signaling is both orthodromic and antidromic, extracellular stimulation may lack specificity. For example, stimulating neocortical layer (L) 4 to recruit ascending inputs to L2/3 pyramidal cells (PCs) may also antidromically activate presynaptic L2/3 PCs, as their descending axons traverse L4. This contaminates L4→L2/3 activation with L2/3→L2/3 responses, which is problematic since these pathways have different properties. Using 2-photon calcium imaging, we found L2/3 PCs that responded to L4 stimulation even after synaptic blockade, demonstrating antidromic activation. Our findings highlight limitations of extracellular stimulation specificity in neocortex.
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Registered trials
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