Evidence map›Paper›PMID 40510131›Full record

ArticleiScience2025

KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells.

Caleb M Dillingham, Harshini Cormaty, Ellen C Morgan, Andrew I Tak, Dakarai E Esgdaille, Paul L Boutz, Rupa Sridharan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Caleb M DillinghamWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Harshini CormatyWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Ellen C MorganWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Andrew I TakWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Dakarai E EsgdailleDepartment of Biochemistry and Biophysics, Center for RNA Biology, Wilmot Cancer Center, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
Paul L BoutzDepartment of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
Rupa SridharanWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.

Funding

Histone Demethylase Control of Post Implantation DevelopmentR01HD105151 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI Rupa Sridharan · 2022 to 2026
$1.9M
Genomics-based approaches to understanding mechanistic alterations of spliceosome function in disease statesR01GM141544 · NIGMS · UNIVERSITY OF ROCHESTER · PI BOUTZ, PAUL LAWRENCE · 2021 to 2025
$1.6M
Histone demethylase control of primordial germ cell specificationF31HD113347 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI Harshini Cormaty · 2024 to 2026
$125k
NICHD NIH HHS F31 HD113347NICHD NIH HHS R01 HD105151NIGMS NIH HHS R01 GM141544
6 · The paper itself

Abstract

Histone modifying enzymes are crucial in preserving cell identity by establishing a conducive chromatin environment for lineage specific transcription factor activity. Mouse pluripotent embryonic stem cells (mESCs) show lower levels of gene repression associated with histone modifications, facilitating rapid response to differentiation cues. The KDM3 family of histone demethylases removes repressive histone H3 lysine 9 dimethylation (H3K9me2). We uncover a surprising role for the KDM3 proteins in the post-transcriptional regulation of mESCs. Proteomic analysis shows KDM3A and KDM3B interacting with RNA processing factors such as EFTUD2 and PRMT5. Acute degradation of the endogenous KDM3A and KDM3B proteins resulted in altered splicing independent of H3K9me2 status or catalytic activity. These splicing changes partially resemble the splicing pattern of the more blastocyst-like ground state of pluripotency and occur in important chromatin and transcription factors such as

Indexed as

Model organismMolecular mechanism of gene regulationProteomicsStem cells researchTranscriptomics

Identifiers

PMID40510131
PMCPMC12159957

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.