Evidence map›Paper›PMID 40509990›Full record

ArticleCirculation research2025

Early Stage Morphogenesis of Transverse Tubules in Rat Cardiomyocytes: The Role of pBIN1.

Qian-Jin Guo, Jing-Hui Liang, Hong-Tao Li, Lin-Lin Li, Yingna Guo, Li-Peng Wang, Xin-Lei Ma, Xian-Jin Xie, Tingting Hou, Shi-Qiang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qian-Jin Guo *State Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).ORCID 0000-0002-0335-2381
Jing-Hui Liang *State Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).ORCID 0009-0007-7899-9781
Hong-Tao Li *State Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).
Lin-Lin Li *State Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).
Yingna GuoState Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).
Li-Peng WangState Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).
Xin-Lei MaState Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).ORCID 0009-0009-5760-5899
Xian-Jin XieDivision of Research and Graduate Programs, Florida State University College of Medicine, Tallahassee, FL (X.-J.X.).
Tingting HouState Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).ORCID 0009-0008-2418-6707
Shi-Qiang WangState Key Laboratory of Membrane Biology, National Biomedical Imaging Center, and College of Life Sciences, Peking University, Beijing, China (Q.-J.G., J.-H.L., H.-T.L., L.-L.L., Y.G., L.-P.W., X.-L.M., T.H., S.-Q.W.).ORCID 0000-0001-7784-4461

Funding

E-C coupling efficiency in hibernation and heart failureR01TW007269 · FIC · PEKING UNIVERSITY · PI WANG, SHI-QIANG · 2005 to 2009
$260k
FIC NIH HHS R01 TW007269
6 · The paper itself

Abstract

backgroundTransverse tubules (TTs) are tubular invaginations of myocyte membrane forming junctions with sarcoplasmic reticulum and are essential for excitation-contraction coupling. Although it is known that TTs begin to develop 2 weeks after birth in rodent cardiomyocytes, the spatial profile and molecular mechanisms of TT morphogenesis are not clear. Understanding the molecular mechanism of TT morphogenesis may provide potential solutions for TT loss in pathogenic conditions such as hypertrophy and heart failure.

methodsTo understand early stage morphogenesis of cardiac TTs, we utilized a scanning electron microscope equipped with a focused ion beam to reconstruct a 3-dimensional spatial profile of developing TT network in cardiomyocytes from 2-week-old rats. We created tamoxifen-inducible cardiac-specific knockout rats to explore the role of exons 11 and 13 of the

resultsWe found that TTs began to develop as intracellular membrane hubs around Z-discs, from which pseudopod-like tubules budded in a relatively random way toward different directions without necessarily connecting to the cell surface. A tubule network forms when membrane branches from adjacent hubs are interconnected. Cardiac-specific knockout of

conclusionsTTs start to develop as budding tubules branching from membrane hubs around Z-discs. This process depends at least partially on the tubulation function of pBIN1 (BIN1 isoforms with PIBM [Bin1+11 and Bin1+11+17]), which is constitutively expressed in rat and human cardiomyocytes. Defective TT morphogenesis due to altered

Indexed as

Adaptor Proteins, Signal TransducingMorphogenesisMyocytes, CardiacTumor Suppressor ProteinsAnimalsExonsRatsRats, Sprague-DawleySarcoplasmic ReticulumAdaptor Proteins, Signal TransducingTumor Suppressor Proteinscardiomyopathy, dilatedmorphogenesismyocytes, cardiacorgan specificityprotein isoforms

Identifiers

PMID40509990
PMCPMC12270754

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.