Evidence map›Paper›PMID 40509691›Full record

ArticleJournal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG2025

Skin microbiome analysis of a junctional epidermolysis bullosa patient treated with genetically modified stem cells.

Alexander Dermietzel, Burcu Tosun, Mathilde Nguyen, Kai Wessel, Luise Rauer, Avidan U Neumann, Tobias Hirsch, CK‐CARE study group, Claudia Traidl-Hoffmann, Matthias Reiger and 2 more

Abstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Skin microbiome analysis of a junctional epidermolysis bullosa patient treated with genetically modified stem cells.Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Alexander DermietzelDepartment of Plastic Surgery, University Hospital Muenster, Muenster, Germany.
Burcu TosunDepartment for Plastic, Reconstructive, and Aesthetic Surgery, Hand Surgery Rotenburg, Rotenburg, Germany.
Mathilde NguyenInstitute of environmental medicine and integrative health, Faculty of Medicine, University of Augsburg, Augsburg, Germany.
Kai WesselDepartment of Plastic Surgery, University Hospital Muenster, Muenster, Germany.
Luise RauerInstitute of environmental medicine and integrative health, Faculty of Medicine, University of Augsburg, Augsburg, Germany.
Avidan U NeumannInstitute of environmental medicine and integrative health, Faculty of Medicine, University of Augsburg, Augsburg, Germany.
Tobias HirschDepartment of Plastic Surgery, University Hospital Muenster, Muenster, Germany.
CK‐CARE study groupCK CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland.
Claudia Traidl-HoffmannInstitute of environmental medicine and integrative health, Faculty of Medicine, University of Augsburg, Augsburg, Germany.
Matthias ReigerInstitute of environmental medicine and integrative health, Faculty of Medicine, University of Augsburg, Augsburg, Germany.
Claudia HülpüschInstitute of environmental medicine and integrative health, Faculty of Medicine, University of Augsburg, Augsburg, Germany.ORCID https://orcid.org/0000-0002-5711-1356
Maximilian KueckelhausDepartment of Plastic Surgery, University Hospital Muenster, Muenster, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectiveJunctional epidermolysis bullosa (JEB) is a subtype of epidermolysis bullosa caused by mutations in the LAMB3 gene. We treated a patient with JEB using genetically corrected autologous epidermal cultures retrovirally transduced with the functional LAMB3 gene sequence. The objective of this study was to analyze the skin microbiome of this patient, with a particular focus on transgenic skin, and to compare the findings to the skin microbiome of healthy controls and patients with atopic dermatitis and well-documented microbial dysbiosis. PATIENTS AND

methodsSkin microbiome analysis was performed on a JEB patient 72 months after combined gene and stem cell therapy. Skin swabs from age-matched healthy controls and atopic dermatitis patients were included from the ProRaD study of CK-CARE.

resultsThe transgenic skin had comparably high relative and absolute Staphylococcus (S.) aureus abundance to blistering and non-blistering skin of the JEB patient, while the total bacterial load was lower. In blistering skin of the JEB patient, higher bacterial load was driven by S. aureus.

conclusionsOur investigation confirms a unique microbiome composition in JEB, characterized by S. aureus driven bacterial overgrowth. The dysbiosis was not reversed in transgenic, non-blistering skin areas. However, the transgenic skin demonstrates stability in an environment of bacterial dysbiosis.

Indexed as

Cell Adhesion MoleculesEpidermolysis Bullosa, JunctionalGenetic TherapyMicrobiotaSkinStem Cell TransplantationDermatitis, AtopicHumansKalininSkin MicrobiomeStaphylococcus aureusCell Adhesion MoleculesKalininJunctional epidermolysis bullosaskin microbiomeStaphylococcus aureus

Identifiers

PMID40509691
PMCPMC12435113

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.