Evidence map›Paper›PMID 40509096›Full record

ArticleAnimals : an open access journal from MDPI2025

Characterization of microRNA and Metabolite Profiles of Seminal Extracellular Vesicles in Boars.

Jianfeng Ma, Shuang Liang, Siyu Chen, Yuqian Shi, Yu Zou, Lei Chen, Lili Niu, Ye Zhao, Yan Wang, Linyuan Shen and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jianfeng MaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Shuang LiangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Siyu ChenState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.ORCID 0009-0004-2260-3934
Yuqian ShiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Yu ZouState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Lei ChenState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Lili NiuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Ye ZhaoState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Yan WangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Linyuan ShenState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Li ZhuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Mailin GanState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.ORCID 0000-0001-9900-3559

Funding

China Agriculture Research System CARS-35China Postdoctoral Science Foundation BX20230250, 2023M732509National Center of Technology Innovation for Pigs NCTIP-XD/C13National Natural Science Foundation of China 32302689Sichuan Science and Technology Program 2021YFYZ0007, 2021ZDZX0008, 2024NSFSC1176The Program for Pig Industry Technology System Innovation Team of Sichuan Province sccxtd-2024-08-09
6 · The paper itself

Abstract

Extracellular vesicles (EVs) contain bioactive substances and mediate a multitude of physiological functions. EVs can be found in most body fluids and are particularly abundant in semen. EVs have the potential to become a biomarker for the quality of boar semen. In this study, EVs were isolated from the semen of relatively young (10 months of age, Y-EVs) and old (30 months of age, O-EVs) duroc boars using ultracentrifugation. The isolated EVs were characterized using a transmission electron microscope, nanoparticle tracking analysis, and Western blotting. MicroRNA (miRNA) profiles and metabolomes were analyzed using high-throughput sequencing and liquid chromatography-mass spectrometry, respectively. The median particle sizes of Y-EVs and O-EVs were 151.3 nm and 162.1 nm, respectively. miR-148a-3p, miR-10b, miR-21-5p, miR-10a-5p, let-7a, etc., were identified as highly enriched miRNAs in seminal EVs of boars. Comparative analysis revealed 41 differentially expressed miRNAs and 132 differential metabolites between Y-EVs and O-EVs. Notably, 18 miRNAs were upregulated in O-EVs, such as miR-339-5p, miR-125a, miR-423-3p, and miR-29c, which were mainly enriched in endocytosis, focal adhesion, and adherens junction. KEGG pathway analysis further indicated that differential metabolites were enriched in glycerophospholipid metabolism. These results provide an insight into the functional roles of seminal EVs.

Indexed as

boarextracellular vesiclemetabolomemiRNAsemen

Identifiers

PMID40509096
PMCPMC12153518

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.