Evidence map›Paper›PMID 40508980›Full record

ArticleAnimals : an open access journal from MDPI2025

Effects of Dietary Docosahexaenoic Acid Levels on the Growth, Body Composition, and Health of Liver and Intestine in Juvenile Tiger Puffer (

Lu Zhang, Chenchen Bian, Ziling Song, Yang Liu, Jiahao Liu, Qiang Ma, Yuliang Wei, Mengqing Liang, Houguo Xu

Abstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lu ZhangCollege of Fisheries and Life Sciences, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China.
Chenchen BianState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.
Ziling SongCollege of Fisheries and Life Sciences, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China.
Yang LiuCollege of Fisheries and Life Sciences, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China.
Jiahao LiuState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.
Qiang MaState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.ORCID 0000-0001-7635-2342
Yuliang WeiState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.ORCID 0000-0001-5032-1580
Mengqing LiangState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.
Houguo XuState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.ORCID 0000-0002-1000-9928

Funding

China Agriculture Research System CARS-47Chinese Academy of Fishery Sciences 2023TD52National Key R&D Program of China 2024YFD2402000Taishan Scholars none
6 · The paper itself

Abstract

Docosahexaenoic acid (DHA), a long-chain polyunsaturated fatty acid, plays a critical role in animal growth, inflammatory regulation, lipid metabolism, and neurological functions. However, the optimal dietary requirement of DHA for tiger puffer remains unknown. This study systematically investigated the effects of different dietary DHA levels on the growth performance, body composition, hematological parameters and tissue physiology of tiger puffer (average initial body weight 17.78 ± 1.92 g). Six experimental diets with graded DHA concentrations (0.09%, 0.57%, 1.35%, 1.61%, 2.28%, and 3.08% dry matter) were formulated. The feeding experiment was carried out in a seawater flow-through system for eight weeks, with each diet assigned to three replicate tanks. Based on the regression analysis of weight gain and specific growth rate, the maximum values were observed at the dietary DHA level of 1.75% and 1.88%, respectively. Appropriate DHA levels also significantly improved the muscle protein synthesis and lipid metabolism, and strengthened the intestinal morphology. Furthermore, a threshold for efficient DHA deposition in muscle was identified, beyond which excess DHA (3.08%) may be β-oxidized and therefore largely wasted. In conclusion, the optimal dietary DHA level for juvenile tiger puffer should be within the range of 1.75-1.88%.

Indexed as

aquafeedfatty acid depositionmarine fishn-3 polyunsaturated fatty acidnutrient requirement

Identifiers

PMID40508980
PMCPMC12153612

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.