Evidence map›Paper›PMID 40508045›Full record

ArticleInternational journal of molecular sciences2025

Targeting of Epithelial Cell Adhesion Molecule-Expressing Malignant Tumors Using an Albumin-Binding Domain-Fused Designed Ankyrin Repeat Protein: Effect of the Molecular Architecture.

Vladimir Tolmachev, Anzhelika Vorobyeva, Alia Hani Binti Rosly, Javad Garousi, Yongsheng Liu, Torbjörn Gräslund, Eleftherios Papalanis, Alexey Schulga, Elena Konovalova, Anna Orlova and 2 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Vladimir TolmachevDepartment of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden.ORCID 0000-0002-6122-1734
Anzhelika VorobyevaDepartment of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden.ORCID 0000-0002-4778-3909
Alia Hani Binti RoslyDepartment of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden.
Javad GarousiDepartment of Protein Science, KTH-Royal Institute of Technology, 106 91 Stockholm, Sweden.
Yongsheng LiuDepartment of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden.ORCID 0000-0001-5871-5779
Torbjörn GräslundDepartment of Protein Science, KTH-Royal Institute of Technology, 106 91 Stockholm, Sweden.ORCID 0000-0002-5391-600X
Eleftherios PapalanisDepartment of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden.ORCID 0009-0006-5340-8618
Alexey SchulgaMolecular Immunology Laboratory, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.ORCID 0000-0003-3425-0828
Elena KonovalovaMolecular Immunology Laboratory, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Anna OrlovaDepartment of Medicinal Chemistry, Uppsala University, 751 83 Uppsala, Sweden.ORCID 0000-0001-6120-2683
Sergey M DeyevMolecular Immunology Laboratory, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.ORCID 0000-0002-3952-0631
Maryam OroujeniDepartment of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden.ORCID 0000-0003-2660-9837

Funding

Ministry of Science and Higher Education of the Russian Federation 075-15-2024-536Svenska Sällskapet för Medicinsk Forskning PG-22-0350-H-01Swedish Cancer Society 20 0893 Pj, 20 0893 Pj , 20 0893 Pj, 20 0815 PjF, 20 0815 PjFSwedish Research Council 2019-00986, 2023-02158, 2022-01519Uppsala University UFV-PA 2022/3323
6 · The paper itself

Abstract

Designed ankyrin repeat protein (DARPin) Ec1, a small scaffold protein (18 kDa), binds with high affinity the epithelial cell adhesion molecule (EpCAM) that is overexpressed in several carcinomas. To enhance the targeted delivery of cytotoxic drugs using Ec1, we investigated the potential of fusing Ec1 with an albumin-binding domain (ABD) to improve its circulation time and decrease renal uptake. Two fusion proteins were created, Ec1-ABD, with the ABD at the C-terminus, and ABD-Ec1, with the ABD at the N-terminus. Both variants were labeled with

Indexed as

AlbuminsDesigned Ankyrin Repeat ProteinsEpithelial Cell Adhesion MoleculeNeoplasmsAnimalsBinding SitesCell Line, TumorFemaleGenetic VariationHumansIndium RadioisotopesMiceAlbuminsDesigned Ankyrin Repeat ProteinsEpithelial Cell Adhesion MoleculeIndium-111Indium Radioisotopesalbumin-binding domain (ABD)designed ankyrin repeat proteinepithelial cell adhesion molecule (EpCAM)fusion proteinnude micexenograft

Identifiers

PMID40508045
PMCPMC12154114

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.