Evidence map›Paper›PMID 40508035›Full record

ArticleInternational journal of molecular sciences2025

Impact of DNA Extraction and 16S rRNA Gene Amplification Strategy on Microbiota Profiling of Faecal Samples.

Francesca Toto, Matteo Scanu, Maurizio Gramegna, Lorenza Putignani, Federica Del Chierico

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Francesca TotoUnit of Microbiome, Bambino Gesù Children's Hospital, IRCCS, 00144 Rome, Italy.ORCID 0000-0002-8976-9013
Matteo ScanuUnit of Microbiome, Bambino Gesù Children's Hospital, IRCCS, 00144 Rome, Italy.ORCID 0000-0001-6266-2884
Maurizio GramegnaTechnogenetics S.p.A., 26900 Lodi, Italy.
Lorenza PutignaniUnit of Microbiomics and Unit of Microbiome, Bambino Gesù Children's Hospital, IRCCS, 00144 Rome, Italy.ORCID 0000-0003-0134-2830
Federica Del ChiericoUnit of Microbiome, Bambino Gesù Children's Hospital, IRCCS, 00144 Rome, Italy.ORCID 0000-0002-4204-4736

Funding

Italian Ministry of Health Current Research fundsTechnogenetics S.p.A. n.a.
6 · The paper itself

Abstract

High-throughput 16S rRNA metagenomic sequencing has advanced our understanding of the gut microbiome, but its reliability depends on upstream processes such as DNA extraction and bacterial library preparation. In this study, we evaluated the impact of three different DNA extraction methods (a manual method with an ad hoc-designed pre-extraction phase (PE-QIA), and two automated magnetic bead-based methods (T180H and TAT132H)) and two bacterial library preparation protocols (

Indexed as

DNA, BacterialFecesGastrointestinal MicrobiomeMicrobiotaRNA, Ribosomal, 16SBacteriaHigh-Throughput Nucleotide SequencingHumansMetagenomeMetagenomicsDNA, BacterialRNA, Ribosomal, 16S16S rRNA metagenomicsbacterial library preparationDNA extractionfaecal samplegut microbiotaPCR-based sequencing

Identifiers

PMID40508035
PMCPMC12154973

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.