Evidence map›Paper›PMID 40508011›Full record

ArticleInternational journal of molecular sciences2025

Design, Co-Expression, and Evaluation for Assembly of the Structural Proteins from Thermophilic Bacteriophage ΦIN93.

Hong Liu, Milad Kheirvari, Ebenezer Tumban

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Multi-gene Co-expression systems inSynthetic and systems biotechnology · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hong LiuGraduate Program in One Health Sciences, School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA.
Milad KheirvariGraduate Program in One Health Sciences, School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA.ORCID 0000-0003-3385-6019
Ebenezer TumbanGraduate Program in One Health Sciences, School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA.

Funding

Development of a novel and broadly applicable thermostable bacteriophage VLPs platforms for vaccine design, drug delivery, and imagingR15AI146982 · NIAID · TEXAS TECH UNIVERSITY · PI TUMBAN, EBENEZER · 2020 to 2020
$444k
NIAID NIH HHS R15 AI146982USA National Institutes of Health 7R15AI146982-02
6 · The paper itself

Abstract

Bacteriophage ΦIN93 has an icosahedral-like capsid that is believed to be composed of two putative capsid or coat proteins, namely open reading frame (ORF)13 and ORF14. In addition to the two capsid proteins, there are other proteins that may be associated with the structure of the virus. For example, five other proteins (ORF12, ORF16, ORF17, ORF19, and ORF20) in the virus have been identified as putative membrane-associated proteins. It is believed that membrane-associated proteins associate with coat proteins (serve as scaffolding proteins) to promote viral assembly. While the expression/co-expression of ORF13 and ORF14 have been done to assess if they can assemble to form virus-like particles (VLPs), the expression of any of the membrane-associated proteins and their contribution to assembly have never been attempted. In this study, we successfully co-expressed, for the first time, three membrane-associated proteins (ORF12, ORF16, ORF17) in addition to ORF13 and ORF14 in thermophilic bacteria (

Indexed as

BacteriophagesViral Structural ProteinsVirus AssemblyCapsid ProteinsEscherichia coliGene ExpressionOpen Reading FramesThermus thermophilusCapsid ProteinsViral Structural ProteinsBL21 Starcoat proteinsmembrane-associated proteinsprotein co-expressionthermophilic bacteriathermophilic bacteriophage ΦIN93

Identifiers

PMID40508011
PMCPMC12154516

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.